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Title: A candidate gene for fire blight resistance in Malus × robusta 5 is coding for a CC-NBS-LRR
Authors : Fahrentrapp, Johannes
Broggini, Giovanni A. L.
Kellerhals, Markus
Peil, Andreas
Richter, Klaus
Zini, Elena
Gessler, Cesare
Published in : Tree Genetics & Genomes
Volume(Issue) : 9
Issue : 1
Pages : 237
Pages to: 251
Publisher / Ed. Institution : Springer
Issue Date: Feb-2013
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Resistance gene; Apple; Weinbau; Fire blight
Subject (DDC) : 634: Orchards, fruits and forestry
Abstract: Fire blight is the most important bacterial disease in apple (Malus ×  domestica) and pear (Pyrus communis) production. Today, the causal bacterium Erwinia amylovora is present in many apple- and pear-growing areas. We investigated the natural resistance of the wild apple Malus ×  robusta 5 against E. amylovora, previously mapped to linkage group 3. With a fine-mapping approach on a population of 2,133 individuals followed by phenotyping of the recombinants from the region of interest, we developed flanking markers useful for marker-assisted selection. Open reading frames were predicted on the sequence of a BAC spanning the resistance locus. One open reading frame coded for a protein belonging to the NBS–LRR family. The in silico investigation of the structure of the candidate resistance gene against fire blight of M. ×  robusta 5, FB_MR5, led us hypothesize the presence of a coiled-coil region followed by an NBS and an LRR-like structure with the consensus ‘LxxLx[IL]xxCxxLxxL’. The function of FB_MR5 was predicted in agreement with the decoy/guard model, that FB_MR5 monitors the transcribed RIN4_MR5, a homolog of RIN4 of Arabidopsis thaliana that could interact with the previously described effector AvrRpt2EA of E. amylovora.
Further description : Erworben im Rahmen der Schweizer Nationallizenzen (
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific Journal
DOI : 10.1007/s11295-012-0550-3
ISSN: 1614-2942
Appears in Collections:Publikationen Life Sciences und Facility Management

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