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dc.contributor.authorImseng, Nicole-
dc.contributor.authorSteiger, Nina-
dc.contributor.authorFrasson, David-
dc.contributor.authorSievers, Martin-
dc.contributor.authorTappe, Alexander-
dc.contributor.authorGreller, Gerhard-
dc.contributor.authorEibl, Dieter-
dc.contributor.authorEibl-Schindler, Regine-
dc.date.accessioned2018-03-14T10:13:11Z-
dc.date.available2018-03-14T10:13:11Z-
dc.date.issued2014-05-22-
dc.identifier.issn1618-0240de_CH
dc.identifier.issn1618-2863de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/3740-
dc.description.abstractSpodoptera frugiperda-9 (Sf-9) cells used in conjunction with the baculovirus expression vector system (BEVS) represent a promising platformfor the rapid development and manufacture of protein complexes and virus-like particle (VLP) products. Several studies have described the superiority of single-use wave-mixed bioreactors although reusable stirred and, more recently, single-use stirred bioreactors have also been successfully applied. Due to their bioengineering characteristics (more homogeneous energy dissipation, reduced foam formation), wave-mixed systems are often preferred. However, a direct comparison of the influence of single-use wave-mixed and single-use stirred bioreactors on cell growth and protein expression in Sf-9/BEVS-based production processes was still lacking. We investigated Sf-9 cell growth and expression of a recombinant secreted alkaline phosphatase (rSEAP) in thewave-mixed BIOSTAT® RMaswell as the stirred UniVessel R SU and a serum-free culture medium. Irrespective of the bioreactor system, comparable growth, substrate, and metabolite courses as well as peak cell densities (>1.2 × 107 cells mL−1) were observed in Sf-9 cell expansions performed in batch mode. Additionally, identical rSEAP quality and maximumrSEAP activities were found in biphasic productions in both bioreactor systems. Concluding, comparability of single-use wave-mixed and stirred bioreactors for insect cell culture processes was demonstrated for the first time. de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofEngineering in Life Sciencesde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleSingle-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scalede_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.publisher.placeWeinheimde_CH
dc.identifier.doi10.1002/elsc.201300131de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end271de_CH
zhaw.pages.start264de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume14de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Imseng, N., Steiger, N., Frasson, D., Sievers, M., Tappe, A., Greller, G., Eibl, D., & Eibl-Schindler, R. (2014). Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale. Engineering in Life Sciences, 14(3), 264–271. https://doi.org/10.1002/elsc.201300131
Imseng, N. et al. (2014) ‘Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale’, Engineering in Life Sciences, 14(3), pp. 264–271. Available at: https://doi.org/10.1002/elsc.201300131.
N. Imseng et al., “Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale,” Engineering in Life Sciences, vol. 14, no. 3, pp. 264–271, May 2014, doi: 10.1002/elsc.201300131.
IMSENG, Nicole, Nina STEIGER, David FRASSON, Martin SIEVERS, Alexander TAPPE, Gerhard GRELLER, Dieter EIBL und Regine EIBL-SCHINDLER, 2014. Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale. Engineering in Life Sciences. 22 Mai 2014. Bd. 14, Nr. 3, S. 264–271. DOI 10.1002/elsc.201300131
Imseng, Nicole, Nina Steiger, David Frasson, Martin Sievers, Alexander Tappe, Gerhard Greller, Dieter Eibl, and Regine Eibl-Schindler. 2014. “Single-Use Wave-Mixed versus Stirred Bioreactors for Insect-Cell/BEVS-Based Protein Expression at Benchtop Scale.” Engineering in Life Sciences 14 (3): 264–71. https://doi.org/10.1002/elsc.201300131.
Imseng, Nicole, et al. “Single-Use Wave-Mixed versus Stirred Bioreactors for Insect-Cell/BEVS-Based Protein Expression at Benchtop Scale.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 264–71, https://doi.org/10.1002/elsc.201300131.


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