Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Imseng, Nicole | - |
dc.contributor.author | Steiger, Nina | - |
dc.contributor.author | Frasson, David | - |
dc.contributor.author | Sievers, Martin | - |
dc.contributor.author | Tappe, Alexander | - |
dc.contributor.author | Greller, Gerhard | - |
dc.contributor.author | Eibl, Dieter | - |
dc.contributor.author | Eibl-Schindler, Regine | - |
dc.date.accessioned | 2018-03-14T10:13:11Z | - |
dc.date.available | 2018-03-14T10:13:11Z | - |
dc.date.issued | 2014-05-22 | - |
dc.identifier.issn | 1618-0240 | de_CH |
dc.identifier.issn | 1618-2863 | de_CH |
dc.identifier.uri | https://digitalcollection.zhaw.ch/handle/11475/3740 | - |
dc.description.abstract | Spodoptera frugiperda-9 (Sf-9) cells used in conjunction with the baculovirus expression vector system (BEVS) represent a promising platformfor the rapid development and manufacture of protein complexes and virus-like particle (VLP) products. Several studies have described the superiority of single-use wave-mixed bioreactors although reusable stirred and, more recently, single-use stirred bioreactors have also been successfully applied. Due to their bioengineering characteristics (more homogeneous energy dissipation, reduced foam formation), wave-mixed systems are often preferred. However, a direct comparison of the influence of single-use wave-mixed and single-use stirred bioreactors on cell growth and protein expression in Sf-9/BEVS-based production processes was still lacking. We investigated Sf-9 cell growth and expression of a recombinant secreted alkaline phosphatase (rSEAP) in thewave-mixed BIOSTAT® RMaswell as the stirred UniVessel R SU and a serum-free culture medium. Irrespective of the bioreactor system, comparable growth, substrate, and metabolite courses as well as peak cell densities (>1.2 × 107 cells mL−1) were observed in Sf-9 cell expansions performed in batch mode. Additionally, identical rSEAP quality and maximumrSEAP activities were found in biphasic productions in both bioreactor systems. Concluding, comparability of single-use wave-mixed and stirred bioreactors for insect cell culture processes was demonstrated for the first time. | de_CH |
dc.language.iso | en | de_CH |
dc.publisher | Wiley | de_CH |
dc.relation.ispartof | Engineering in Life Sciences | de_CH |
dc.rights | Licence according to publishing contract | de_CH |
dc.subject.ddc | 660: Technische Chemie | de_CH |
dc.title | Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale | de_CH |
dc.type | Beitrag in wissenschaftlicher Zeitschrift | de_CH |
dcterms.type | Text | de_CH |
zhaw.departement | Life Sciences und Facility Management | de_CH |
zhaw.organisationalunit | Institut für Chemie und Biotechnologie (ICBT) | de_CH |
zhaw.publisher.place | Weinheim | de_CH |
dc.identifier.doi | 10.1002/elsc.201300131 | de_CH |
zhaw.funding.eu | No | de_CH |
zhaw.issue | 3 | de_CH |
zhaw.originated.zhaw | Yes | de_CH |
zhaw.pages.end | 271 | de_CH |
zhaw.pages.start | 264 | de_CH |
zhaw.publication.status | publishedVersion | de_CH |
zhaw.volume | 14 | de_CH |
zhaw.publication.review | Peer review (Publikation) | de_CH |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Imseng, N., Steiger, N., Frasson, D., Sievers, M., Tappe, A., Greller, G., Eibl, D., & Eibl-Schindler, R. (2014). Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale. Engineering in Life Sciences, 14(3), 264–271. https://doi.org/10.1002/elsc.201300131
Imseng, N. et al. (2014) ‘Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale’, Engineering in Life Sciences, 14(3), pp. 264–271. Available at: https://doi.org/10.1002/elsc.201300131.
N. Imseng et al., “Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale,” Engineering in Life Sciences, vol. 14, no. 3, pp. 264–271, May 2014, doi: 10.1002/elsc.201300131.
IMSENG, Nicole, Nina STEIGER, David FRASSON, Martin SIEVERS, Alexander TAPPE, Gerhard GRELLER, Dieter EIBL und Regine EIBL-SCHINDLER, 2014. Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale. Engineering in Life Sciences. 22 Mai 2014. Bd. 14, Nr. 3, S. 264–271. DOI 10.1002/elsc.201300131
Imseng, Nicole, Nina Steiger, David Frasson, Martin Sievers, Alexander Tappe, Gerhard Greller, Dieter Eibl, and Regine Eibl-Schindler. 2014. “Single-Use Wave-Mixed versus Stirred Bioreactors for Insect-Cell/BEVS-Based Protein Expression at Benchtop Scale.” Engineering in Life Sciences 14 (3): 264–71. https://doi.org/10.1002/elsc.201300131.
Imseng, Nicole, et al. “Single-Use Wave-Mixed versus Stirred Bioreactors for Insect-Cell/BEVS-Based Protein Expression at Benchtop Scale.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 264–71, https://doi.org/10.1002/elsc.201300131.
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