| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale |
| Autor/-in: | Imseng, Nicole Steiger, Nina Frasson, David Sievers, Martin Tappe, Alexander Greller, Gerhard Eibl, Dieter Eibl-Schindler, Regine |
| DOI: | 10.1002/elsc.201300131 |
| Erschienen in: | Engineering in Life Sciences |
| Band(Heft): | 14 |
| Heft: | 3 |
| Seite(n): | 264 |
| Seiten bis: | 271 |
| Erscheinungsdatum: | 22-Mai-2014 |
| Verlag / Hrsg. Institution: | Wiley |
| Verlag / Hrsg. Institution: | Weinheim |
| ISSN: | 1618-0240 1618-2863 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 660: Technische Chemie |
| Zusammenfassung: | Spodoptera frugiperda-9 (Sf-9) cells used in conjunction with the baculovirus expression vector system (BEVS) represent a promising platformfor the rapid development and manufacture of protein complexes and virus-like particle (VLP) products. Several studies have described the superiority of single-use wave-mixed bioreactors although reusable stirred and, more recently, single-use stirred bioreactors have also been successfully applied. Due to their bioengineering characteristics (more homogeneous energy dissipation, reduced foam formation), wave-mixed systems are often preferred. However, a direct comparison of the influence of single-use wave-mixed and single-use stirred bioreactors on cell growth and protein expression in Sf-9/BEVS-based production processes was still lacking. We investigated Sf-9 cell growth and expression of a recombinant secreted alkaline phosphatase (rSEAP) in thewave-mixed BIOSTAT® RMaswell as the stirred UniVessel R SU and a serum-free culture medium. Irrespective of the bioreactor system, comparable growth, substrate, and metabolite courses as well as peak cell densities (>1.2 × 107 cells mL−1) were observed in Sf-9 cell expansions performed in batch mode. Additionally, identical rSEAP quality and maximumrSEAP activities were found in biphasic productions in both bioreactor systems. Concluding, comparability of single-use wave-mixed and stirred bioreactors for insect cell culture processes was demonstrated for the first time. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/3740 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Imseng, N., Steiger, N., Frasson, D., Sievers, M., Tappe, A., Greller, G., Eibl, D., & Eibl-Schindler, R. (2014). Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale. Engineering in Life Sciences, 14(3), 264–271. https://doi.org/10.1002/elsc.201300131
Imseng, N. et al. (2014) ‘Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale’, Engineering in Life Sciences, 14(3), pp. 264–271. Available at: https://doi.org/10.1002/elsc.201300131.
N. Imseng et al., “Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale,” Engineering in Life Sciences, vol. 14, no. 3, pp. 264–271, May 2014, doi: 10.1002/elsc.201300131.
IMSENG, Nicole, Nina STEIGER, David FRASSON, Martin SIEVERS, Alexander TAPPE, Gerhard GRELLER, Dieter EIBL und Regine EIBL-SCHINDLER, 2014. Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale. Engineering in Life Sciences. 22 Mai 2014. Bd. 14, Nr. 3, S. 264–271. DOI 10.1002/elsc.201300131
Imseng, Nicole, Nina Steiger, David Frasson, Martin Sievers, Alexander Tappe, Gerhard Greller, Dieter Eibl, and Regine Eibl-Schindler. 2014. “Single-Use Wave-Mixed versus Stirred Bioreactors for Insect-Cell/BEVS-Based Protein Expression at Benchtop Scale.” Engineering in Life Sciences 14 (3): 264–71. https://doi.org/10.1002/elsc.201300131.
Imseng, Nicole, et al. “Single-Use Wave-Mixed versus Stirred Bioreactors for Insect-Cell/BEVS-Based Protein Expression at Benchtop Scale.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 264–71, https://doi.org/10.1002/elsc.201300131.
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