Title: Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale
Authors : Imseng, Nicole
Steiger, Nina
Frasson, David
Sievers, Martin
Tappe, Alexander
Greller, Gerhard
Eibl, Dieter
Eibl-Schindler, Regine
Published in : Engineering in Life Sciences
Volume(Issue) : 14
Issue : 3
Pages : 264
Pages to: 271
Publisher / Ed. Institution : Wiley
Publisher / Ed. Institution: Weinheim
Issue Date: 22-May-2014
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 660: Chemical engineering
Abstract: Spodoptera frugiperda-9 (Sf-9) cells used in conjunction with the baculovirus expression vector system (BEVS) represent a promising platformfor the rapid development and manufacture of protein complexes and virus-like particle (VLP) products. Several studies have described the superiority of single-use wave-mixed bioreactors although reusable stirred and, more recently, single-use stirred bioreactors have also been successfully applied. Due to their bioengineering characteristics (more homogeneous energy dissipation, reduced foam formation), wave-mixed systems are often preferred. However, a direct comparison of the influence of single-use wave-mixed and single-use stirred bioreactors on cell growth and protein expression in Sf-9/BEVS-based production processes was still lacking. We investigated Sf-9 cell growth and expression of a recombinant secreted alkaline phosphatase (rSEAP) in thewave-mixed BIOSTAT® RMaswell as the stirred UniVessel R SU and a serum-free culture medium. Irrespective of the bioreactor system, comparable growth, substrate, and metabolite courses as well as peak cell densities (>1.2 × 107 cells mL−1) were observed in Sf-9 cell expansions performed in batch mode. Additionally, identical rSEAP quality and maximumrSEAP activities were found in biphasic productions in both bioreactor systems. Concluding, comparability of single-use wave-mixed and stirred bioreactors for insect cell culture processes was demonstrated for the first time. 
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.1002/elsc.201300131
ISSN: 1618-0240
URI: https://digitalcollection.zhaw.ch/handle/11475/3740
Appears in Collections:Publikationen Life Sciences und Facility Management

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