Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale

Imseng, Nicole; Steiger, Nina; Frasson, David; Sievers, Martin; Tappe, Alexander; Greller, Gerhard; Eibl, Dieter; Eibl-Schindler, Regine

doi:10.1002/elsc.201300131

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale
Authors:	Imseng, Nicole Steiger, Nina Frasson, David Sievers, Martin Tappe, Alexander Greller, Gerhard Eibl, Dieter Eibl-Schindler, Regine
DOI:	10.1002/elsc.201300131
Published in:	Engineering in Life Sciences
Volume(Issue):	14
Issue:	3
Page(s):	264
Pages to:	271
Issue Date:	22-May-2014
Publisher / Ed. Institution:	Wiley
Publisher / Ed. Institution:	Weinheim
ISSN:	1618-0240 1618-2863
Language:	English
Subject (DDC):	660: Chemical engineering
Abstract:	Spodoptera frugiperda-9 (Sf-9) cells used in conjunction with the baculovirus expression vector system (BEVS) represent a promising platformfor the rapid development and manufacture of protein complexes and virus-like particle (VLP) products. Several studies have described the superiority of single-use wave-mixed bioreactors although reusable stirred and, more recently, single-use stirred bioreactors have also been successfully applied. Due to their bioengineering characteristics (more homogeneous energy dissipation, reduced foam formation), wave-mixed systems are often preferred. However, a direct comparison of the influence of single-use wave-mixed and single-use stirred bioreactors on cell growth and protein expression in Sf-9/BEVS-based production processes was still lacking. We investigated Sf-9 cell growth and expression of a recombinant secreted alkaline phosphatase (rSEAP) in thewave-mixed BIOSTAT® RMaswell as the stirred UniVessel R SU and a serum-free culture medium. Irrespective of the bioreactor system, comparable growth, substrate, and metabolite courses as well as peak cell densities (>1.2 × 107 cells mL−1) were observed in Sf-9 cell expansions performed in batch mode. Additionally, identical rSEAP quality and maximumrSEAP activities were found in biphasic productions in both bioreactor systems. Concluding, comparability of single-use wave-mixed and stirred bioreactors for insect cell culture processes was demonstrated for the first time.
URI:	https://digitalcollection.zhaw.ch/handle/11475/3740
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

There are no files associated with this item.

Show full item record

Imseng, N., Steiger, N., Frasson, D., Sievers, M., Tappe, A., Greller, G., Eibl, D., & Eibl-Schindler, R. (2014). Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale. Engineering in Life Sciences, 14(3), 264–271. https://doi.org/10.1002/elsc.201300131

Imseng, N. et al. (2014) ‘Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale’, Engineering in Life Sciences, 14(3), pp. 264–271. Available at: https://doi.org/10.1002/elsc.201300131.

N. Imseng et al., “Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale,” Engineering in Life Sciences, vol. 14, no. 3, pp. 264–271, May 2014, doi: 10.1002/elsc.201300131.

IMSENG, Nicole, Nina STEIGER, David FRASSON, Martin SIEVERS, Alexander TAPPE, Gerhard GRELLER, Dieter EIBL und Regine EIBL-SCHINDLER, 2014. Single-use wave-mixed versus stirred bioreactors for insect-cell/BEVS-based protein expression at benchtop scale. Engineering in Life Sciences. 22 Mai 2014. Bd. 14, Nr. 3, S. 264–271. DOI 10.1002/elsc.201300131

Imseng, Nicole, Nina Steiger, David Frasson, Martin Sievers, Alexander Tappe, Gerhard Greller, Dieter Eibl, and Regine Eibl-Schindler. 2014. “Single-Use Wave-Mixed versus Stirred Bioreactors for Insect-Cell/BEVS-Based Protein Expression at Benchtop Scale.” Engineering in Life Sciences 14 (3): 264–71. https://doi.org/10.1002/elsc.201300131.

Imseng, Nicole, et al. “Single-Use Wave-Mixed versus Stirred Bioreactors for Insect-Cell/BEVS-Based Protein Expression at Benchtop Scale.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 264–71, https://doi.org/10.1002/elsc.201300131.