Title: Validated method for the analysis of frangulins A and B and glucofrangulins A and B using HPLC and UHPLC
Authors : Rosenthal, Martin Immanuel
Wolfram, Evelyn
Peter, Samuel
Meier, Beat
Published in : Journal of Natural Products
Volume(Issue) : 77
Issue : 3
Pages : 489
Pages to: 496
Publisher / Ed. Institution : American Chemical Society
Issue Date: 2014
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 615: Pharmacology and therapeutics
Abstract: In the present study, robust and validated HPLC and UHPLC methods for the quantitative determination of frangulins A and B (3 and 4) and glucofrangulins A and B (1 and 2) in the bark of Frangula alnus have been developed. The HPLC method allowed the separation of the analytes in 25 min and the UHPLC method in just 13 min. The HPLC method used an MN Nucleodur C 18 125 × 4 mm column with 3 μm particles, while the UHPLC method used a Waters Acquity UPLC BEH C18, 100 × 2.1 mm column with 1.7 μm particles. Mobile phase A consisted of water and 1.25 mL/L phosphoric acid (85%), while mobile phase B consisted of CH3CN/MeOH (20:80). The flow rates were set to 1mL/min for the HPLC method and 0.4 mL/min for the UHPLC method, with the column temperature held at 50 °C and the detection wavelength being 435 nm for either method. A fractional factorial design was used to check the robustness of the methods. The resolution of the analytes was never less than 1.5 when the factors were varied in the tested range. The conditions for ultrasonic extraction were optimized by response surface methodology and found to be 68% CH 3 CN in the extraction solvent, 35°C extraction temperature, and a duration of 25 min. The extraction procedure was determined to be very robust against small deviations of these factors.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1021/np400736s
ISSN: 0163-3864
URI: https://digitalcollection.zhaw.ch/handle/11475/3049
Appears in Collections:Publikationen Life Sciences und Facility Management

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