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dc.contributor.authorThürer, Reto-
dc.contributor.authorVigassy, Tamás-
dc.contributor.authorHirayama, Martina-
dc.contributor.authorWang, Joseph-
dc.contributor.authorBakker, Eric-
dc.contributor.authorPretsch, Ernö-
dc.date.accessioned2018-02-26T07:55:34Z-
dc.date.available2018-02-26T07:55:34Z-
dc.date.issued2007-05-26-
dc.identifier.issn0003-2700de_CH
dc.identifier.issn1520-6882de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/3018-
dc.description.abstractPotentiometric sensors based on polymer membrane electrodes, if properly optimized, are useful for measurements at trace levels. The expected independence of the electrochemical signal of the sample size makes them extremely attractive for measurements in small volumes. Here, we report on electrodes for the potentiometric detection of cadmium ions that reach a detection limit of 6 nM and utilize a Na+-selective electrode as pseudoreference in order to facilitate measurements in 150-µL samples. A potentiometric immunoassay of mouse IgG is performed via CdSe quantum dot labels on a secondary antibody according to a sandwich immunoassay protocol in a microtiter plate format. The CdSe quantum dots are found to be easily dissolved/oxidized in a matter of minutes with hydrogen peroxide, allowing us to maintain the pH at a near-neutral value. The potentiometric protein immunoassay exhibits a log-linear response ranging from 0.15 to 4.0 pmol of IgG, with a detection limit of <10 fmol in 150-µL sample wells.de_CH
dc.language.isoende_CH
dc.publisherAmerican Chemical Societyde_CH
dc.relation.ispartofAnalytical Chemistryde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectImmunoassayde_CH
dc.subjectPotentiometricde_CH
dc.subjectQuantum dotde_CH
dc.subject.ddc540: Chemiede_CH
dc.titlePotentiometric Immunoassay with Quantum Dot Labelsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.publisher.placeWashingtonde_CH
dc.identifier.doi10.1021/ac070932mde_CH
zhaw.funding.euNode_CH
zhaw.issue13de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end5110de_CH
zhaw.pages.start5107de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume79de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in Collections:Publikationen School of Engineering

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