Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Potentiometric Immunoassay with Quantum Dot Labels
Authors: Thürer, Reto
Vigassy, Tamás
Hirayama, Martina
Wang, Joseph
Bakker, Eric
Pretsch, Ernö
DOI: 10.1021/ac070932m
Published in: Analytical Chemistry
Volume(Issue): 79
Issue: 13
Pages: 5107
Pages to: 5110
Issue Date: 26-May-2007
Publisher / Ed. Institution: American Chemical Society
Publisher / Ed. Institution: Washington
ISSN: 0003-2700
Language: English
Subjects: Immunoassay; Potentiometric; Quantum dot
Subject (DDC): 540: Chemistry
Abstract: Potentiometric sensors based on polymer membrane electrodes, if properly optimized, are useful for measurements at trace levels. The expected independence of the electrochemical signal of the sample size makes them extremely attractive for measurements in small volumes. Here, we report on electrodes for the potentiometric detection of cadmium ions that reach a detection limit of 6 nM and utilize a Na+-selective electrode as pseudoreference in order to facilitate measurements in 150-µL samples. A potentiometric immunoassay of mouse IgG is performed via CdSe quantum dot labels on a secondary antibody according to a sandwich immunoassay protocol in a microtiter plate format. The CdSe quantum dots are found to be easily dissolved/oxidized in a matter of minutes with hydrogen peroxide, allowing us to maintain the pH at a near-neutral value. The potentiometric protein immunoassay exhibits a log-linear response ranging from 0.15 to 4.0 pmol of IgG, with a detection limit of <10 fmol in 150-µL sample wells.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: School of Engineering
Appears in Collections:Publikationen School of Engineering

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