| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Potentiometric Immunoassay with Quantum Dot Labels |
| Authors: | Thürer, Reto Vigassy, Tamás Hirayama, Martina Wang, Joseph Bakker, Eric Pretsch, Ernö |
| DOI: | 10.1021/ac070932m |
| Published in: | Analytical Chemistry |
| Volume(Issue): | 79 |
| Issue: | 13 |
| Page(s): | 5107 |
| Pages to: | 5110 |
| Issue Date: | 26-May-2007 |
| Publisher / Ed. Institution: | American Chemical Society |
| Publisher / Ed. Institution: | Washington |
| ISSN: | 0003-2700 1520-6882 |
| Language: | English |
| Subjects: | Immunoassay; Potentiometric; Quantum dot |
| Subject (DDC): | 540: Chemistry |
| Abstract: | Potentiometric sensors based on polymer membrane electrodes, if properly optimized, are useful for measurements at trace levels. The expected independence of the electrochemical signal of the sample size makes them extremely attractive for measurements in small volumes. Here, we report on electrodes for the potentiometric detection of cadmium ions that reach a detection limit of 6 nM and utilize a Na+-selective electrode as pseudoreference in order to facilitate measurements in 150-µL samples. A potentiometric immunoassay of mouse IgG is performed via CdSe quantum dot labels on a secondary antibody according to a sandwich immunoassay protocol in a microtiter plate format. The CdSe quantum dots are found to be easily dissolved/oxidized in a matter of minutes with hydrogen peroxide, allowing us to maintain the pH at a near-neutral value. The potentiometric protein immunoassay exhibits a log-linear response ranging from 0.15 to 4.0 pmol of IgG, with a detection limit of <10 fmol in 150-µL sample wells. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/3018 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | School of Engineering |
| Appears in collections: | Publikationen School of Engineering |
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Thürer, R., Vigassy, T., Hirayama, M., Wang, J., Bakker, E., & Pretsch, E. (2007). Potentiometric Immunoassay with Quantum Dot Labels. Analytical Chemistry, 79(13), 5107–5110. https://doi.org/10.1021/ac070932m
Thürer, R. et al. (2007) ‘Potentiometric Immunoassay with Quantum Dot Labels’, Analytical Chemistry, 79(13), pp. 5107–5110. Available at: https://doi.org/10.1021/ac070932m.
R. Thürer, T. Vigassy, M. Hirayama, J. Wang, E. Bakker, and E. Pretsch, “Potentiometric Immunoassay with Quantum Dot Labels,” Analytical Chemistry, vol. 79, no. 13, pp. 5107–5110, May 2007, doi: 10.1021/ac070932m.
THÜRER, Reto, Tamás VIGASSY, Martina HIRAYAMA, Joseph WANG, Eric BAKKER und Ernö PRETSCH, 2007. Potentiometric Immunoassay with Quantum Dot Labels. Analytical Chemistry. 26 Mai 2007. Bd. 79, Nr. 13, S. 5107–5110. DOI 10.1021/ac070932m
Thürer, Reto, Tamás Vigassy, Martina Hirayama, Joseph Wang, Eric Bakker, and Ernö Pretsch. 2007. “Potentiometric Immunoassay with Quantum Dot Labels.” Analytical Chemistry 79 (13): 5107–10. https://doi.org/10.1021/ac070932m.
Thürer, Reto, et al. “Potentiometric Immunoassay with Quantum Dot Labels.” Analytical Chemistry, vol. 79, no. 13, May 2007, pp. 5107–10, https://doi.org/10.1021/ac070932m.
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