Title: HPTLC methods for identification of green tea and green tea extract
Authors : Reich, Eike
Schibli, Anne
Widmer, Valeria
Jorns, Ruth
Wolfram, Evelyn
DeBatt, Alison
Published in : Journal of Liquid Chromatography & Related Technologies
Volume(Issue) : 29
Issue : 14
Pages : 2141
Pages to: 2151
Publisher / Ed. Institution : Taylor & Francis Inc.
Issue Date: 2007
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Camellia sinensis; Fingerprint; Flavonoids; Green tea; Polyphenols; Validation
Subject (DDC) : 664: Food technology
Abstract: For centuries, the leaves of Camellia sinensis have been used to make green, black, and special types of tea; it has been the basis for one of the most popular beverages all over the world. More recently, green tea has become the raw material for extracts which are used in various beverages, health foods, dietary supplements, and cosmetic items. In view of the Good Manufacturing Practice (cGMP) for dietary supplements, mandating proper identification of raw materials and finished products, it is very important to have the necessary analytical tools at hand. This paper presents HPTLC methods for identification of green tea and green tea extract. On silica gel 60 with ethyl formate, toluene, formic acid, water (30:1.5:4:3) as mobile phase, the flavonoid fingerprint of green tea can give information about the geographical origin of the material. The mobile phase toluene, acetone, formic acid (9:9:2) allows the discrimination of green tea from black and other specialty teas, based on the polyphenol pattern. The latter method has been validated, addressing specificity, stability, reproducibility, and robustness. For additional quality control of extracts, ethyl acetate, methanol, and water (20:2.7:2) can be used as mobile phase to investigate the alkaloid profile, whereas, 1‐butanol, acetone, acetic acid, water (7:7:2:4) provides an amino acid profile.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1080/15512160600760293
ISSN: 1082-6076
URI: https://digitalcollection.zhaw.ch/handle/11475/3015
Appears in Collections:Publikationen Life Sciences und Facility Management

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