Please use this identifier to cite or link to this item:
https://doi.org/10.21256/zhaw-1685| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro |
| Authors: | Michel, Christian Herzog, Simon de Capitani, Christian Burkhardt-Holm, Patricia Pietsch, Constanze |
| DOI: | 10.21256/zhaw-1685 10.1371/journal.pone.0100856 |
| Published in: | PLOS ONE |
| Volume(Issue): | 9 |
| Issue: | 7 |
| Issue Date: | 2014 |
| Publisher / Ed. Institution: | Public Library of Science |
| ISSN: | 1932-6203 |
| Language: | English |
| Subjects: | Animals; Cell Count; Cell Survival; Cells, Cultured; Epithelial Cells; Gills; Minerals; Oncorhynchus mykiss |
| Subject (DDC): | 571: Physiology and related subjects 630: Agriculture |
| Abstract: | Worldwide increases in fluvial fine sediment are a threat to aquatic animal health. Fluvial fine sediment is always a mixture of particles whose mineralogical composition differs depending on the sediment source and catchment area geology. Nonetheless, whether particle impact in aquatic organisms differs between mineral species remains to be investigated. This study applied an in vitro approach to evaluate cytotoxicity and uptake of four common fluvial mineral particles (quartz, feldspar, mica, and kaolin; concentrations: 10, 50, 250 mg L(-1)) in the rainbow trout epithelial gill cell line RTgill-W1. Cells were exposed for 24, 48, 72, and 96 h. Cytotoxicity assays for cell membrane integrity (propidium iodide assay), oxidative stress (H2DCF-DA assay), and metabolic activity (MTT assay) were applied. These assays were complemented with cell counts and transmission electron microscopy. Regardless of mineral species, particles ≤ 2 µm in diameter were taken up by the cells, suggesting that particles of all mineral species came into contact and interacted with the cells. Not all particles, however, caused strong cytotoxicity: Among all assays the tectosilicates quartz and feldspar caused sporadic maximum changes of 0.8-1.2-fold compared to controls. In contrast, cytotoxicity of the clay particles was distinctly stronger and even differed between the two particle types: mica induced concentration-dependent increases in free radicals, with consistent 1.6-1.8-fold-changes at the 250 mg L(-1) concentration, and a dilated endoplasmic reticulum. Kaolin caused concentration-dependent increases in cell membrane damage, with consistent 1.3-1.6-fold increases at the 250 mg L(-1) concentration. All effects occurred in the presence or absence of 10% fetal bovine serum. Cell numbers per se were marginally affected. Results indicate that (i.) natural mineral particles can be cytotoxic to gill epithelial cells, (ii.) their cytotoxic potential differs between mineral species, with clay particles being more cytotoxic, and (iii.) some clays might induce effects comparable to engineered nanoparticles. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/2849 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2014_Pietsch_Natural_Mineral_Particles_PLOS_one.pdf | 940.16 kB | Adobe PDF |  View/Open |
Show full item record
Michel, C., Herzog, S., de Capitani, C., Burkhardt-Holm, P., & Pietsch, C. (2014). Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro. Plos One, 9(7). https://doi.org/10.21256/zhaw-1685
Michel, C. et al. (2014) ‘Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro’, PLOS ONE, 9(7). Available at: https://doi.org/10.21256/zhaw-1685.
C. Michel, S. Herzog, C. de Capitani, P. Burkhardt-Holm, and C. Pietsch, “Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro,” PLOS ONE, vol. 9, no. 7, 2014, doi: 10.21256/zhaw-1685.
MICHEL, Christian, Simon HERZOG, Christian DE CAPITANI, Patricia BURKHARDT-HOLM und Constanze PIETSCH, 2014. Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro. PLOS ONE. 2014. Bd. 9, Nr. 7. DOI 10.21256/zhaw-1685
Michel, Christian, Simon Herzog, Christian de Capitani, Patricia Burkhardt-Holm, and Constanze Pietsch. 2014. “Natural Mineral Particles Are Cytotoxic to Rainbow Trout Gill Epithelial Cells in Vitro.” Plos One 9 (7). https://doi.org/10.21256/zhaw-1685.
Michel, Christian, et al. “Natural Mineral Particles Are Cytotoxic to Rainbow Trout Gill Epithelial Cells in Vitro.” Plos One, vol. 9, no. 7, 2014, https://doi.org/10.21256/zhaw-1685.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.