A qPCR-based, population dynamics approach for the development of a bacteriophage-based biopesticide

Gayder, Steven; Kammerecker, Sandrine; Gervásio, Kellen; Henriques, André; Torres-Cortés, Gloria; Kőrösiné Papp, Szilvia; Kovács, Tamás; Raffai, Krisztina; Cabezón Largas, Sixto; de Santos Prieto, Borja; Fieseler, Lars

Publication type:	Conference other
Type of review:	Peer review (abstract)
Title:	A qPCR-based, population dynamics approach for the development of a bacteriophage-based biopesticide
Authors:	Gayder, Steven Kammerecker, Sandrine Gervásio, Kellen Henriques, André Torres-Cortés, Gloria Kőrösiné Papp, Szilvia Kovács, Tamás Raffai, Krisztina Cabezón Largas, Sixto de Santos Prieto, Borja Fieseler, Lars
et. al:	No
Conference details:	Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022
Issue Date:	6-Sep-2022
Language:	English
Subjects:	Bacteriophage; qPCR; Population dynamics; Biopesticide
Subject (DDC):	579: Microbiology
Abstract:	Bacteriophages are viruses that target specific bacteria and kill them through their own replicative life cycle. As more countries ban the use of antibiotics for the control of fire blight and antibiotic resistant strains of Erwinia amylovora become more widespread, the use of phages as biological control agents is rapidly gaining interest. In the multinational Horizon 2020 Project PhageFire, several academic and industry partners have teamed up to develop a phage-based product for the control of fire blight. To design an effective and affordable phage formulation, the choice of phages in the cocktail is critical. The ultimate goal is to maximize synergistic interactions and minimize antagonistic interactions between the different phages while using the fewest number of phages possible to reduce production costs. To achieve this goal, we used a quantitative real-time PCR (qPCR) approach to study different combinations of a collection of phages against a combined library of E. amylovora strains representative of the pathogen diversity in Europe. With qPCR, the populations of the phages and E. amylovora can be measured individually over time in liquid cultures. This allows us to determine which phages synergize best together, while eliminating those that get outcompeted and add little overall value to the cocktail. With this work, in conjunction with additional product formulation, regulatory efforts, and field trials we aim to develop an effective, affordable, phage-based biopesticide for the control of fire blight.
URI:	https://digitalcollection.zhaw.ch/handle/11475/27324
Fulltext version:	Published version
License (according to publishing contract):	Not specified
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Food and Beverage Innovation (ILGI)
Published as part of the ZHAW project:	PhageFire – An effective and environmentally friendly solution to control fire blight disease caused by Erwinia amylovora in pome fruit crops
Appears in collections:	Publikationen Life Sciences und Facility Management

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Gayder, S., Kammerecker, S., Gervásio, K., Henriques, A., Torres-Cortés, G., Kőrösiné Papp, S., Kovács, T., Raffai, K., Cabezón Largas, S., de Santos Prieto, B., & Fieseler, L. (2022, September 6). A qPCR-based, population dynamics approach for the development of a bacteriophage-based biopesticide. Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022.

Gayder, S. et al. (2022) ‘A qPCR-based, population dynamics approach for the development of a bacteriophage-based biopesticide’, in Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022.

S. Gayder et al., “A qPCR-based, population dynamics approach for the development of a bacteriophage-based biopesticide,” in Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022, Sep. 2022.

GAYDER, Steven, Sandrine KAMMERECKER, Kellen GERVÁSIO, André HENRIQUES, Gloria TORRES-CORTÉS, Szilvia KŐRÖSINÉ PAPP, Tamás KOVÁCS, Krisztina RAFFAI, Sixto CABEZÓN LARGAS, Borja DE SANTOS PRIETO und Lars FIESELER, 2022. A qPCR-based, population dynamics approach for the development of a bacteriophage-based biopesticide. In: Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022. Conference presentation. 6 September 2022

Gayder, Steven, Sandrine Kammerecker, Kellen Gervásio, André Henriques, Gloria Torres-Cortés, Szilvia Kőrösiné Papp, Tamás Kovács, et al. 2022. “A qPCR-Based, Population Dynamics Approach for the Development of a Bacteriophage-Based Biopesticide.” Conference presentation. In Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022.

Gayder, Steven, et al. “A qPCR-Based, Population Dynamics Approach for the Development of a Bacteriophage-Based Biopesticide.” Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022, 2022.