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|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography|
|Authors:||Bergmann, Alan J.|
Vermeirssen, Etiënne L.M.
|Published in:||Food Packaging and Shelf Life|
|Publisher / Ed. Institution:||Elsevier|
|Subjects:||Food contact materials (FCM); High-performance thin layer chromatography (HPTLC); Genotoxicity; Threshold of toxicological concern (TTC); Paperboard; Non-intentionally added substances (NIAS)|
|Subject (DDC):||664: Food technology|
|Abstract:||High-performance thin-layer chromatography (HPTLC)-bioassays are promising new methods for detecting bioactive chemicals in food packaging. Here, we test whether direct-acting genotoxic chemicals are detectable in food contact materials (FCM) using HPTLC-bioassays. First, an interactive worksheet lays out steps to calculate needed detection limits in (bio)analytical methods from regulatory limits, including thresholds of toxicological concern (TTC). Second, we show that the sensitivity of a HPTLC-genotoxicity assay to low doses of chemicals, including food contact chemicals, is greater than a standardized microtiter plate version and in vitro assays already reported. Third, using HPTLC, we detected genotoxicity in extracts of FCM, and not in simulated migrates of FCM. Applying the worksheet to calculate needed detection limits in FCM migrates, we observed that seven of ten genotoxic chemicals would be detectable with HPTLC if present at the regulatory 10 ppb limit and two of ten at TTC for adults. With development, HPTLC-bioassays might become the best option for supporting safety assessment of genotoxicants in food packaging.|
|Fulltext version:||Published version|
|License (according to publishing contract):||CC BY 4.0: Attribution 4.0 International|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Natural Resource Sciences (IUNR)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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Bergmann, A. J., Breitenbach, M., Muñoz, C., Simon, E., McCombie, G., Biedermann, M., Schönborn, A., & Vermeirssen, E. L. M. (2023). Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography. Food Packaging and Shelf Life, 36(101052). https://doi.org/10.1016/j.fpsl.2023.101052
Bergmann, A.J. et al. (2023) ‘Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography’, Food Packaging and Shelf Life, 36(101052). Available at: https://doi.org/10.1016/j.fpsl.2023.101052.
A. J. Bergmann et al., “Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography,” Food Packaging and Shelf Life, vol. 36, no. 101052, Apr. 2023, doi: 10.1016/j.fpsl.2023.101052.
Bergmann, Alan J., et al. “Towards Detecting Genotoxic Chemicals in Food Packaging at Thresholds of Toxicological Concern Using Bioassays with High-Performance Thin-Layer Chromatography.” Food Packaging and Shelf Life, vol. 36, no. 101052, Apr. 2023, https://doi.org/10.1016/j.fpsl.2023.101052.
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