Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography

Bergmann, Alan J.; Breitenbach, Milena; Muñoz, Celine; Simon, Eszter; McCombie, Gregor; Biedermann, Maurus; Schönborn, Andreas; Vermeirssen, Etiënne L.M.

doi:10.1016/j.fpsl.2023.101052

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-27078

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography
Authors:	Bergmann, Alan J. Breitenbach, Milena Muñoz, Celine Simon, Eszter McCombie, Gregor Biedermann, Maurus Schönborn, Andreas Vermeirssen, Etiënne L.M.
et. al:	No
DOI:	10.1016/j.fpsl.2023.101052 10.21256/zhaw-27078
Published in:	Food Packaging and Shelf Life
Volume(Issue):	36
Issue:	101052
Issue Date:	Apr-2023
Publisher / Ed. Institution:	Elsevier
ISSN:	2214-2894
Language:	English
Subjects:	Food contact materials (FCM); High-performance thin layer chromatography (HPTLC); Genotoxicity; Threshold of toxicological concern (TTC); Paperboard; Non-intentionally added substances (NIAS)
Subject (DDC):	664: Food technology
Abstract:	High-performance thin-layer chromatography (HPTLC)-bioassays are promising new methods for detecting bioactive chemicals in food packaging. Here, we test whether direct-acting genotoxic chemicals are detectable in food contact materials (FCM) using HPTLC-bioassays. First, an interactive worksheet lays out steps to calculate needed detection limits in (bio)analytical methods from regulatory limits, including thresholds of toxicological concern (TTC). Second, we show that the sensitivity of a HPTLC-genotoxicity assay to low doses of chemicals, including food contact chemicals, is greater than a standardized microtiter plate version and in vitro assays already reported. Third, using HPTLC, we detected genotoxicity in extracts of FCM, and not in simulated migrates of FCM. Applying the worksheet to calculate needed detection limits in FCM migrates, we observed that seven of ten genotoxic chemicals would be detectable with HPTLC if present at the regulatory 10 ppb limit and two of ten at TTC for adults. With development, HPTLC-bioassays might become the best option for supporting safety assessment of genotoxicants in food packaging.
URI:	https://digitalcollection.zhaw.ch/handle/11475/27078
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Bergmann, A. J., Breitenbach, M., Muñoz, C., Simon, E., McCombie, G., Biedermann, M., Schönborn, A., & Vermeirssen, E. L. M. (2023). Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography. Food Packaging and Shelf Life, 36(101052). https://doi.org/10.1016/j.fpsl.2023.101052

Bergmann, A.J. et al. (2023) ‘Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography’, Food Packaging and Shelf Life, 36(101052). Available at: https://doi.org/10.1016/j.fpsl.2023.101052.

A. J. Bergmann et al., “Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography,” Food Packaging and Shelf Life, vol. 36, no. 101052, Apr. 2023, doi: 10.1016/j.fpsl.2023.101052.

BERGMANN, Alan J., Milena BREITENBACH, Celine MUÑOZ, Eszter SIMON, Gregor MCCOMBIE, Maurus BIEDERMANN, Andreas SCHÖNBORN und Etiënne L.M. VERMEIRSSEN, 2023. Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography. Food Packaging and Shelf Life. April 2023. Bd. 36, Nr. 101052. DOI 10.1016/j.fpsl.2023.101052

Bergmann, Alan J., Milena Breitenbach, Celine Muñoz, Eszter Simon, Gregor McCombie, Maurus Biedermann, Andreas Schönborn, and Etiënne L.M. Vermeirssen. 2023. “Towards Detecting Genotoxic Chemicals in Food Packaging at Thresholds of Toxicological Concern Using Bioassays with High-Performance Thin-Layer Chromatography.” Food Packaging and Shelf Life 36 (101052). https://doi.org/10.1016/j.fpsl.2023.101052.

Bergmann, Alan J., et al. “Towards Detecting Genotoxic Chemicals in Food Packaging at Thresholds of Toxicological Concern Using Bioassays with High-Performance Thin-Layer Chromatography.” Food Packaging and Shelf Life, vol. 36, no. 101052, Apr. 2023, https://doi.org/10.1016/j.fpsl.2023.101052.