Bovine neutrophil chemotaxis to Listeria monocytogenes in neurolisteriosis depends on microglia-released rather than bacterial factors

Bagatella, Stefano; Haghayegh Jahromi, Neda; Monney, Camille; Polidori, Margherita; Gall, Flavio Max; Marchionatti, Emma; Serra, Fabienne; Riedl, Rainer; Engelhardt, Britta; Oevermann, Anna

doi:10.1186/s12974-022-02653-1

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26823

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Bovine neutrophil chemotaxis to Listeria monocytogenes in neurolisteriosis depends on microglia-released rather than bacterial factors
Authors:	Bagatella, Stefano Haghayegh Jahromi, Neda Monney, Camille Polidori, Margherita Gall, Flavio Max Marchionatti, Emma Serra, Fabienne Riedl, Rainer Engelhardt, Britta Oevermann, Anna
et. al:	No
DOI:	10.1186/s12974-022-02653-1 10.21256/zhaw-26823
Published in:	Journal of Neuroinflammation
Volume(Issue):	19
Issue:	304
Issue Date:	16-Dec-2022
Publisher / Ed. Institution:	BioMed Central
ISSN:	1742-2094
Language:	English
Subjects:	Formyl peptide; IL-8; Microglia; Neuroinfection; Neurolisteriosis; Neutrophil; Human; Microglia; Chemotaxis; Interleukin-8; Chemotaxis, leukocyte; Listeria monocytogene
Subject (DDC):	579: Microbiology 616: Internal medicine and diseases
Abstract:	Background: Listeria monocytogenes (Lm) is a bacterial pathogen of major concern for humans and ruminants due to its neuroinvasive potential and its ability to cause deadly encephalitis (neurolisteriosis). On one hand, polymorphonuclear neutrophils (PMN) are key players in the defense against Lm, but on the other hand intracerebral infiltration with PMN is associated with significant neural tissue damage. Lm-PMN interactions in neurolisteriosis are poorly investigated, and factors inducing PMN chemotaxis to infectious foci containing Lm in the central nervous system (CNS) remain unidentified. Methods: In this study, we assessed bovine PMN chemotaxis towards Lm and supernatants of infected endogenous brain cell populations in ex vivo chemotaxis assays, to identify chemotactic stimuli for PMN chemotaxis towards Lm in the brain. In addition, microglial secretion of IL-8 was assessed both ex vivo and in situ. Results: Our data show that neither Lm cell wall components nor intact bacteria elicit chemotaxis of bovine PMN ex vivo. Moreover, astrocytes and neural cells fail to induce bovine PMN chemotaxis upon infection. In contrast, supernatant from Lm infected microglia readily induced chemotaxis of bovine PMN. Microglial expression and secretion of IL-8 was identified during early Lm infection in vitro and in situ, although IL-8 blocking with a specific antibody could not abrogate PMN chemotaxis towards Lm infected microglial supernatant. Conclusions: These data provide evidence that host-derived rather than bacterial factors trigger PMN chemotaxis to bacterial foci in the CNS, that microglia have a primary role as initiators of bovine PMN chemotaxis into the brain during neurolisteriosis and that blockade of these factors could be a therapeutic target to limit intrathecal PMN chemotaxis and PMN associated damage in neurolisteriosis.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26823
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Bagatella, S., Haghayegh Jahromi, N., Monney, C., Polidori, M., Gall, F. M., Marchionatti, E., Serra, F., Riedl, R., Engelhardt, B., & Oevermann, A. (2022). Bovine neutrophil chemotaxis to Listeria monocytogenes in neurolisteriosis depends on microglia-released rather than bacterial factors. Journal of Neuroinflammation, 19(304). https://doi.org/10.1186/s12974-022-02653-1

Bagatella, S. et al. (2022) ‘Bovine neutrophil chemotaxis to Listeria monocytogenes in neurolisteriosis depends on microglia-released rather than bacterial factors’, Journal of Neuroinflammation, 19(304). Available at: https://doi.org/10.1186/s12974-022-02653-1.

S. Bagatella et al., “Bovine neutrophil chemotaxis to Listeria monocytogenes in neurolisteriosis depends on microglia-released rather than bacterial factors,” Journal of Neuroinflammation, vol. 19, no. 304, Dec. 2022, doi: 10.1186/s12974-022-02653-1.

BAGATELLA, Stefano, Neda HAGHAYEGH JAHROMI, Camille MONNEY, Margherita POLIDORI, Flavio Max GALL, Emma MARCHIONATTI, Fabienne SERRA, Rainer RIEDL, Britta ENGELHARDT und Anna OEVERMANN, 2022. Bovine neutrophil chemotaxis to Listeria monocytogenes in neurolisteriosis depends on microglia-released rather than bacterial factors. Journal of Neuroinflammation. 16 Dezember 2022. Bd. 19, Nr. 304. DOI 10.1186/s12974-022-02653-1

Bagatella, Stefano, Neda Haghayegh Jahromi, Camille Monney, Margherita Polidori, Flavio Max Gall, Emma Marchionatti, Fabienne Serra, Rainer Riedl, Britta Engelhardt, and Anna Oevermann. 2022. “Bovine Neutrophil Chemotaxis to Listeria Monocytogenes in Neurolisteriosis Depends on Microglia-Released rather than Bacterial Factors.” Journal of Neuroinflammation 19 (304). https://doi.org/10.1186/s12974-022-02653-1.

Bagatella, Stefano, et al. “Bovine Neutrophil Chemotaxis to Listeria Monocytogenes in Neurolisteriosis Depends on Microglia-Released rather than Bacterial Factors.” Journal of Neuroinflammation, vol. 19, no. 304, Dec. 2022, https://doi.org/10.1186/s12974-022-02653-1.