Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material

Knobloch, Marco C.; Schinkel, Lena; Kohler, Hans-Peter E.; Mathis, Flurin; Kern, Susanne; Bleiner, Davide; Heeb, Norbert V.

doi:10.1016/j.chemosphere.2021.131199

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26804

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material
Authors:	Knobloch, Marco C. Schinkel, Lena Kohler, Hans-Peter E. Mathis, Flurin Kern, Susanne Bleiner, Davide Heeb, Norbert V.
et. al:	No
DOI:	10.1016/j.chemosphere.2021.131199 10.21256/zhaw-26804
Published in:	Chemosphere
Volume(Issue):	283
Issue:	131199
Issue Date:	2021
Publisher / Ed. Institution:	Elsevier
ISSN:	0045-6535 1879-1298
Language:	English
Subjects:	Chlorinated olefins (COs); Chlorinated paraffins (CPs); Enzymatic dechlorination; First-order kinetic model; Persistent organic pollutants (POPs); Alkenes; Environmental Monitoring; Kinetics; Paraffin; Hydrocarbons, Chlorinated; Sphingomonadaceae
Subject (DDC):	660: Chemical engineering
Abstract:	Structure, reactivity and physico-chemical properties of polyhalogenated compounds determine their up-take, transport, bio-accumulation, transformation and toxicity and their environmental fate. In technical mixtures of chlorinated paraffins (CPs), these properties are distributed due to the presence of thousands of homologues. We hypothesized that roles of CP dehalogenation reactions, catalyzed by the haloalkane dehalogenase LinB, depend on structural properties of the substrates, e.g. chlorination degree and carbon-chain length. We exposed mixtures of chlorinated undecanes, dodecanes and tridecanes in-vitro to LinB from Sphingobium Indicum bacteria. These single-chain CP-materials also contain small amounts of chlorinated olefins (COs), which can be distinct by mathematical deconvolution of respective mass-spectra. With this procedure, we obtained homologue-specific transformation kinetics of substrates differing in saturation degree, chlorination degree and carbon chain-length. For all homologues, two-stage first-order kinetic models were established, which described the faster conversion of reactive material and the slower transformation of more persistent material. Half-lifes of 0.5-3.2 h and 56-162 h were determined for more reactive and more persistent CP-material. Proportions of persistent material increased steadily from 18 to 67% for lower (Cl6) to higher (Cl11) chlorinated paraffins and olefins. Conversion efficiencies decreased with increasing chlorination degree from 97 to 70%. Carbon-chain length had only minor effects on transformation rates. Hence, the conversion was faster and more efficient for lower-chlorinated material, and slower for higher-chlorinated and longer-chained CPs and COs. Current legislation has banned short-chain chlorinated paraffins (SCCPs) and forced a transition to longer-chain CPs. This may be counterproductive with regard to enzymatic transformation with LinB.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26804
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Knobloch, M. C., Schinkel, L., Kohler, H.-P. E., Mathis, F., Kern, S., Bleiner, D., & Heeb, N. V. (2021). Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material. Chemosphere, 283(131199). https://doi.org/10.1016/j.chemosphere.2021.131199

Knobloch, M.C. et al. (2021) ‘Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material’, Chemosphere, 283(131199). Available at: https://doi.org/10.1016/j.chemosphere.2021.131199.

M. C. Knobloch et al., “Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material,” Chemosphere, vol. 283, no. 131199, 2021, doi: 10.1016/j.chemosphere.2021.131199.

KNOBLOCH, Marco C., Lena SCHINKEL, Hans-Peter E. KOHLER, Flurin MATHIS, Susanne KERN, Davide BLEINER und Norbert V. HEEB, 2021. Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material. Chemosphere. 2021. Bd. 283, Nr. 131199. DOI 10.1016/j.chemosphere.2021.131199

Knobloch, Marco C., Lena Schinkel, Hans-Peter E. Kohler, Flurin Mathis, Susanne Kern, Davide Bleiner, and Norbert V. Heeb. 2021. “Transformation of Short-Chain Chlorinated Paraffins and Olefins with the Bacterial Dehalogenase LinB from Sphingobium Indicum : Kinetic Models for the Homologue-Specific Conversion of Reactive and Persistent Material.” Chemosphere 283 (131199). https://doi.org/10.1016/j.chemosphere.2021.131199.

Knobloch, Marco C., et al. “Transformation of Short-Chain Chlorinated Paraffins and Olefins with the Bacterial Dehalogenase LinB from Sphingobium Indicum : Kinetic Models for the Homologue-Specific Conversion of Reactive and Persistent Material.” Chemosphere, vol. 283, no. 131199, 2021, https://doi.org/10.1016/j.chemosphere.2021.131199.