Influence of the post-harvest storage time on the multi-biological potential, phenolic and pyrrolizidine alkaloid content of comfrey (Symphytum officinale L.) roots collected from different European regions

Trifan, Adriana; Zengin, Gokhan; Sinan, Kouadio Ibrahime; Esslinger, Nils; Grubelnik, Andreas; Wolfram, Evelyn; Skalicka-Woźniak, Krystyna; Minceva, Mirjana; Luca, Simon Vlad

doi:10.3390/plants10091825

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-25891

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Influence of the post-harvest storage time on the multi-biological potential, phenolic and pyrrolizidine alkaloid content of comfrey (Symphytum officinale L.) roots collected from different European regions
Authors:	Trifan, Adriana Zengin, Gokhan Sinan, Kouadio Ibrahime Esslinger, Nils Grubelnik, Andreas Wolfram, Evelyn Skalicka-Woźniak, Krystyna Minceva, Mirjana Luca, Simon Vlad
et. al:	No
DOI:	10.3390/plants10091825 10.21256/zhaw-25891
Published in:	Plants
Volume(Issue):	10
Issue:	9
Page(s):	1825
Issue Date:	2021
Publisher / Ed. Institution:	MDPI
ISSN:	2223-7747
Language:	English
Subjects:	Symphytum; Antioxidant; Enzyme inhibition; Globoidnan A; Rosmarinic acid; Storage
Subject (DDC):	580: Plants (Botany)
Abstract:	Comfrey (Symphytum officinale L.) roots are well-known bioactive ingredients included in various cosmeceutical and pharmaceutical preparations. In this study, the influence of the post-harvest storage on the chemico-biological potential of roots collected from different European regions and stored for up to six months was investigated. Total phenolic content (TPC) and total phenolic acid content (TPAC) were spectrophotometrically estimated, whereas the levels of individual phenolic and pyrrolizidine alkaloidal markers were determined by HPLC-DAD and HPLC-MS/MS, respectively. The changes in the biological potential was tracked via antioxidant (DPPH, ABTS, CUPRAC, and FRAP) and anti-enzymatic (cholinesterase, tyrosinase, glucosidase, and amylase) assays. TPC and TPAC varied from 6.48-16.57 mg GAE/g d.w. root and from 2.67-9.03 mg CAE/g, respectively. The concentration of the four phenolics (rosmarinic acid, globoidnan A, globoidnan B, rabdosiin) and six pyrrolizidine alkaloids generally showed maximum values at 1-3 months, after which their levels significantly decreased. With respect to the bioassays, the samples showed a wide range of antioxidant and anti-enzymatic effects; however, a direct storage time-bioactivity relationship was not observed. Similar conclusions were also revealed by the multivariate and correlation analyses. Our study could improve the current knowledge of the shelf-life properties of comfrey-based products and enhance their industrial exploitation.
URI:	https://digitalcollection.zhaw.ch/handle/11475/25891
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Trifan, A., Zengin, G., Sinan, K. I., Esslinger, N., Grubelnik, A., Wolfram, E., Skalicka-Woźniak, K., Minceva, M., & Luca, S. V. (2021). Influence of the post-harvest storage time on the multi-biological potential, phenolic and pyrrolizidine alkaloid content of comfrey (Symphytum officinale L.) roots collected from different European regions. Plants, 10(9), 1825. https://doi.org/10.3390/plants10091825

Trifan, A. et al. (2021) ‘Influence of the post-harvest storage time on the multi-biological potential, phenolic and pyrrolizidine alkaloid content of comfrey (Symphytum officinale L.) roots collected from different European regions’, Plants, 10(9), p. 1825. Available at: https://doi.org/10.3390/plants10091825.

A. Trifan et al., “Influence of the post-harvest storage time on the multi-biological potential, phenolic and pyrrolizidine alkaloid content of comfrey (Symphytum officinale L.) roots collected from different European regions,” Plants, vol. 10, no. 9, p. 1825, 2021, doi: 10.3390/plants10091825.

TRIFAN, Adriana, Gokhan ZENGIN, Kouadio Ibrahime SINAN, Nils ESSLINGER, Andreas GRUBELNIK, Evelyn WOLFRAM, Krystyna SKALICKA-WOŹNIAK, Mirjana MINCEVA und Simon Vlad LUCA, 2021. Influence of the post-harvest storage time on the multi-biological potential, phenolic and pyrrolizidine alkaloid content of comfrey (Symphytum officinale L.) roots collected from different European regions. Plants. 2021. Bd. 10, Nr. 9, S. 1825. DOI 10.3390/plants10091825

Trifan, Adriana, Gokhan Zengin, Kouadio Ibrahime Sinan, Nils Esslinger, Andreas Grubelnik, Evelyn Wolfram, Krystyna Skalicka-Woźniak, Mirjana Minceva, and Simon Vlad Luca. 2021. “Influence of the Post-Harvest Storage Time on the Multi-Biological Potential, Phenolic and Pyrrolizidine Alkaloid Content of Comfrey (Symphytum Officinale L.) Roots Collected from Different European Regions.” Plants 10 (9): 1825. https://doi.org/10.3390/plants10091825.

Trifan, Adriana, et al. “Influence of the Post-Harvest Storage Time on the Multi-Biological Potential, Phenolic and Pyrrolizidine Alkaloid Content of Comfrey (Symphytum Officinale L.) Roots Collected from Different European Regions.” Plants, vol. 10, no. 9, 2021, p. 1825, https://doi.org/10.3390/plants10091825.