|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Engineering Fe(II)/α-ketoglutarate-dependent halogenases and desaturases|
Buller, Rebecca M.
|Publisher / Ed. Institution:||American Chemical Society|
|Subjects:||Hydrogen abstraction; Organic reaction; Halogenation; Monomer|
|Subject (DDC):||572: Biochemistry|
|Abstract:||Fe(II)/α-ketoglutarate-dependent dioxygenases (α-KGDs) are widespread enzymes in aerobic biology and serve a remarkable array of biological functions, including roles in collagen biosynthesis, plant and animal development, transcriptional regulation, nucleic acid modification, and secondary metabolite biosynthesis. This functional diversity is reflected in the enzymes' catalytic flexibility as α-KGDs can catalyze an intriguing set of synthetically valuable reactions, such as hydroxylations, halogenations, and desaturations, capturing the interest of scientists across disciplines. Mechanistically, all α-KGDs are understood to follow a similar activation pathway to generate a substrate radical, yet how individual members of the enzyme family direct this key intermediate toward the different reaction outcomes remains elusive, triggering structural, computational, spectroscopic, kinetic, and enzyme engineering studies. In this Perspective, we will highlight how first enzyme and substrate engineering examples suggest that the chemical reaction pathway within α-KGDs can be intentionally tailored using rational design principles. We will delineate the structural and mechanistic investigations of the reprogrammed enzymes and how they begin to inform about the enzymes' structure-function relationships that determine chemoselectivity. Application of this knowledge in future enzyme and substrate engineering campaigns will lead to the development of powerful C-H activation catalysts for chemical synthesis.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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Papadopoulou, A., Meyer, F., & Buller, R. M. (2022). Engineering Fe(II)/α-ketoglutarate-dependent halogenases and desaturases. Biochemistry, 62(2), 229–240. https://doi.org/10.1021/acs.biochem.2c00115
Papadopoulou, A., Meyer, F. and Buller, R.M. (2022) ‘Engineering Fe(II)/α-ketoglutarate-dependent halogenases and desaturases’, Biochemistry, 62(2), pp. 229–240. Available at: https://doi.org/10.1021/acs.biochem.2c00115.
A. Papadopoulou, F. Meyer, and R. M. Buller, “Engineering Fe(II)/α-ketoglutarate-dependent halogenases and desaturases,” Biochemistry, vol. 62, no. 2, pp. 229–240, Apr. 2022, doi: 10.1021/acs.biochem.2c00115.
Papadopoulou, Athena, et al. “Engineering Fe(II)/α-Ketoglutarate-Dependent Halogenases and Desaturases.” Biochemistry, vol. 62, no. 2, Apr. 2022, pp. 229–40, https://doi.org/10.1021/acs.biochem.2c00115.
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