Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift
Art der Begutachtung: Peer review (Publikation)
Titel: Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model
Autor/-in: D'Agostino, Stefania
Rimann, Markus
Gamba, Piergiorgio
Perilongo, Giorgio
Pozzobon, Michela
Raghunath, Michael
et. al: No
DOI: 10.1016/j.bprint.2022.e00213
Erschienen in: Bioprinting
Band(Heft): 27
Heft: e00213
Erscheinungsdatum: 30-Mai-2022
Verlag / Hrsg. Institution: Elsevier
ISSN: 2405-8866
Sprache: Englisch
Schlagwörter: Bioprinting; Rhabdomyosarcoma microenvironment; Macromolecular crowding; 3D model of rhabdomyosarcoma
Fachgebiet (DDC): 610.28: Biomedizin, Biomedizinische Technik
Zusammenfassung: The role of the extracellular matrix (ECM) in tumor recurrence and metastasis has been gaining attention. Indeed, not only cellular, but also structural proteins influence migratory and invasive capacity of tumor cells, including growth and resistance to drugs. Therefore, new in vitro tumor models that entail improved ECM formation and deposition are needed. Here, we are developed three-dimensional (3D) models of pediatric soft tissue sarcoma (Rhabdomyosarcoma [RMS]) with the two major subgroups, the embryonal (ERMS) and the alveolar (ARMS) form. We applied macromolecular crowding (MMC) technology to monolayer cultures, spheroids, and 3D bioprinted constructs. In all culture models, exposure to MMC significantly increased ECM deposition. Interestingly, bioprinted constructs showed a collagen and fibronectin matrix architecture that was comparable to that of tumor xenografts. Furthermore, the bioprinted model not only showed tumor cell growth inside the structure but also displayed cell clusters leaving the edges of the bioprinted construct, probably emulating a metastatic mechanism. ARMS and ERMS cells reacted differently in the bioprinted structure. Indeed, the characteristic metastatic behavior was much more pronounced in the more aggressive ARMS subtype. This promising approach opens new avenues for studying RMS microenvironment and creating a platform for cancer drug testing including the native tumor ECM.
Weitere Angaben: EU COST Action CA16119 CellFit
Volltext Version: Publizierte Version
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Chemie und Biotechnologie (ICBT)
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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