Please use this identifier to cite or link to this item:
https://doi.org/10.21256/zhaw-24748| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Bacteriophage S6 requires bacterial cellulose for Erwinia amylovora infection |
| Authors: | Knecht, Leandra E. Heinrich, Nadine Born, Yannick Felder, Katja Pelludat, Cosima Loessner, Martin J. Fieseler, Lars |
| et. al: | Yes |
| DOI: | 10.1111/1462-2920.15973 10.21256/zhaw-24748 |
| Published in: | Environmental Microbiology |
| Volume(Issue): | 24 |
| Issue: | 8 |
| Page(s): | 3436 |
| Pages to: | 3450 |
| Issue Date: | 15-Mar-2022 |
| Publisher / Ed. Institution: | Wiley |
| ISSN: | 1462-2912 1462-2920 |
| Language: | English |
| Subjects: | Bacteriophage |
| Subject (DDC): | 579: Microbiology |
| Abstract: | Bacteriophages are highly selective in targeting bacteria. This selectivity relies on the specific adsorption of phages to the host cell surface. In this study, a Tn5 transposon mutant library of Erwinia amylovora, the causative agent of fire blight, was screened to identify bacterial receptors required for infection by the podovirus S6. Phage S6 was unable to infect mutants with defects in the bacterial cellulose synthase operon (bcs). The Bcs complex produces and secretes bacterial cellulose, an extracellular polysaccharide associated with bacterial biofilms. Deletion of the bcs operon or associated genes (bcsA, bcsC and bcsZ) verified the crucial role of bacterial cellulose for S6 infection. Application of the cellulose binding dye Congo Red blocked infection by S6. We demonstrate that infective S6 virions degraded cellulose and that Gp95, a phage-encoded cellulase, is involved to catalyse the reaction. In planta S6 did not significantly inhibit fire blight symptom development. Moreover, deletion of bcs genes in E. amylovora did not affect bacterial virulence in blossom infections, indicating that sole application of cellulose targeting phages is less appropriate to biologically control E. amylovora. The interplay between cellulose synthesis, host cell infection and maintenance of the host cell population is discussed. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/24748 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY-NC-ND 4.0: Attribution - Non commercial - No derivatives 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Food and Beverage Innovation (ILGI) |
| Published as part of the ZHAW project: | Mechanisms of phage resistance in Erwinia amylovora |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2022_Knecht-etal_Bacteriophage-S6-requires-bacterial-cellulose.pdf | 3.87 MB | Adobe PDF |  View/Open |
Show full item record
Knecht, L. E., Heinrich, N., Born, Y., Felder, K., Pelludat, C., Loessner, M. J., & Fieseler, L. (2022). Bacteriophage S6 requires bacterial cellulose for Erwinia amylovora infection. Environmental Microbiology, 24(8), 3436–3450. https://doi.org/10.1111/1462-2920.15973
Knecht, L.E. et al. (2022) ‘Bacteriophage S6 requires bacterial cellulose for Erwinia amylovora infection’, Environmental Microbiology, 24(8), pp. 3436–3450. Available at: https://doi.org/10.1111/1462-2920.15973.
L. E. Knecht et al., “Bacteriophage S6 requires bacterial cellulose for Erwinia amylovora infection,” Environmental Microbiology, vol. 24, no. 8, pp. 3436–3450, Mar. 2022, doi: 10.1111/1462-2920.15973.
KNECHT, Leandra E., Nadine HEINRICH, Yannick BORN, Katja FELDER, Cosima PELLUDAT, Martin J. LOESSNER und Lars FIESELER, 2022. Bacteriophage S6 requires bacterial cellulose for Erwinia amylovora infection. Environmental Microbiology. 15 März 2022. Bd. 24, Nr. 8, S. 3436–3450. DOI 10.1111/1462-2920.15973
Knecht, Leandra E., Nadine Heinrich, Yannick Born, Katja Felder, Cosima Pelludat, Martin J. Loessner, and Lars Fieseler. 2022. “Bacteriophage S6 Requires Bacterial Cellulose for Erwinia Amylovora Infection.” Environmental Microbiology 24 (8): 3436–50. https://doi.org/10.1111/1462-2920.15973.
Knecht, Leandra E., et al. “Bacteriophage S6 Requires Bacterial Cellulose for Erwinia Amylovora Infection.” Environmental Microbiology, vol. 24, no. 8, Mar. 2022, pp. 3436–50, https://doi.org/10.1111/1462-2920.15973.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.