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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Bacteriophage S6 requires bacterial cellulose for Erwinia amylovora infection
Authors: Knecht, Leandra E.
Heinrich, Nadine
Born, Yannick
Felder, Katja
Pelludat, Cosima
Loessner, Martin J.
Fieseler, Lars
et. al: Yes
DOI: 10.1111/1462-2920.15973
Published in: Environmental Microbiology
Issue Date: 15-Mar-2022
Publisher / Ed. Institution: Wiley
ISSN: 1462-2912
Language: English
Subjects: Bacteriophage
Subject (DDC): 579: Microbiology
Abstract: Bacteriophages are highly selective in targeting bacteria. This selectivity relies on the specific adsorption of phages to the host cell surface. In this study, a Tn5 transposon mutant library of Erwinia amylovora, the causative agent of fire blight, was screened to identify bacterial receptors required for infection by the podovirus S6. Phage S6 was unable to infect mutants with defects in the bacterial cellulose synthase operon (bcs). The Bcs complex produces and secretes bacterial cellulose, an extracellular polysaccharide associated with bacterial biofilms. Deletion of the bcs operon or associated genes (bcsA, bcsC and bcsZ) verified the crucial role of bacterial cellulose for S6 infection. Application of the cellulose binding dye Congo Red blocked infection by S6. We demonstrate that infective S6 virions degraded cellulose and that Gp95, a phage-encoded cellulase, is involved to catalyse the reaction. In planta S6 did not significantly inhibit fire blight symptom development. Moreover, deletion of bcs genes in E. amylovora did not affect bacterial virulence in blossom infections, indicating that sole application of cellulose targeting phages is less appropriate to biologically control E. amylovora. The interplay between cellulose synthesis, host cell infection and maintenance of the host cell population is discussed.
Fulltext version: Published version
License (according to publishing contract): CC BY-NC-ND 4.0: Attribution - Non commercial - No derivatives 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Published as part of the ZHAW project: Mechanisms of phage resistance in Erwinia amylovora
Appears in collections:Publikationen Life Sciences und Facility Management

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