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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Xanthomonas hydrangeae sp. nov., a novel plant pathogen isolated from Hydrangea arborescens
Authors: Dia, Nay C.
Van Vaerenbergh, Johan
Van Malderghem, Cinzia
Blom, Jochen
Smits, Theo H. M.
Cottyn, Bart
Pothier, Joël F.
et. al: No
DOI: 10.1099/ijsem.0.005163
Published in: International Journal of Systematic and Evolutionary Microbiology
Volume(Issue): 71
Issue: 12
Issue Date: 16-Dec-2021
Publisher / Ed. Institution: Microbiology Society
ISSN: 1466-5026
Language: English
Subjects: Xanthomonas; diagnostics; Overall genome-relatedness indices
Subject (DDC): 572: Biochemistry
579: Microbiology
Abstract: This paper describes a novel species isolated in 2011 and 2012 from nursery-grown Hydrangea arborescens cultivars in Flanders, Belgium. After 4 days at 28 °C, the strains yielded yellow, round, convex and mucoid colonies. Pathogenicity of the strains was confirmed on its isolation host, as well as on Hydrangea quercifolia. Analysis using MALDI-TOF MS identified the Hydrangea strains as belonging to the genus Xanthomonas but excluded them from the species Xanthomonas hortorum. A phylogenetic tree based on gyrB confirmed the close relation to X. hortorum. Three fatty acids were dominant in the Hydrangea isolates: anteiso-C15 : 0, iso-C15 : 0 and summed feature 3 (C16 : 1  ω7c/C16 : 1  ω6c). Unlike X. hortorum pathovars, the Hydrangea strains were unable to grow in the presence of lithium chloride and could only weakly utilize d-fructose-6-PO4 and glucuronamide. Phylogenetic characterization based on multilocus sequence analysis and phylogenomic characterization revealed that the strains are close to, yet distinct from, X. hortorum. The genome sequences of the strains had average nucleotide identity values ranging from 94.35-95.19 % and in silico DNA-DNA hybridization values ranging from 55.70 to 59.40 % to genomes of the X. hortorum pathovars. A genomics-based loop-mediated isothermal amplification assay was developed which was specific to the Hydrangea strains for its early detection. A novel species, Xanthomonas hydrangeae sp. nov., is proposed with strain LMG 31884T (=CCOS 1956T) as the type strain.
Fulltext version: Accepted version
License (according to publishing contract): CC BY 1.0: Attribution 1.0 Generic
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project: Xhortomics: Entwicklung von diagnostischen und epidemiologischen Hilfsmitteln für die Xanthomonas hortorum Spezies Gruppe basierend auf OMICs Technologien
Appears in collections:Publikationen Life Sciences und Facility Management

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Dia-etal_Xanthomonas-hydrangeae_IJSEM-Accepted-Version.pdfAccepted Version306 kBAdobe PDFThumbnail
Dia-etal_Xanthomonas-hydrangeae_IJSEM-Supplementary-Material-Accepted-Version.pdfAccepted Supplementary Material582.24 kBAdobe PDFThumbnail
Fig1-Disease-symptoms.png4.27 MBimage/pngThumbnail
Fig2-16S-tree.eps4.45 MBPostscriptView/Open
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Fig4-whole-genome-tree.eps2.02 MBPostscriptView/Open
Fig5-ANI-isDDH.png1.33 MBimage/pngThumbnail

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