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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Determination of culture design spaces in shaken disposable cultivation systems for CHO suspension cell cultures
Authors: Maschke, Rüdiger W.
Seidel, Stefan
Bley, Thomas
Eibl, Regine
Eibl, Dieter
et. al: No
DOI: 10.1016/j.bej.2021.108224
Published in: Biochemical Engineering Journal
Volume(Issue): 177
Page(s): 108224
Issue Date: Jan-2022
Publisher / Ed. Institution: Elsevier
ISSN: 1369-703X
Language: English
Subjects: Erlenmeyer shake flask; Optimum Growth flask; TubeSpin bioreactor; CHO suspension cell culture; Process engineering characterization; Design space
Subject (DDC): 660.6: Biotechnology
Abstract: Processes involving mammalian cell cultures - especially CHO suspension cells - dominate biopharmaceutical manufacturing. These processes are usually developed in small scale orbitally shaken cultivation systems, and thoroughly characterizing these cultivation systems is crucial to their application in research and the subsequent scale-up to production processes. With the knowledge of process engineering parameters such as oxygen transfer rate, mixing time, and power input, in combination with the demands set by the biological production system, biomass growth and product yields can be anticipated and even increased. However, the available data sources for orbitally shaken cultivation systems are often incomplete and thus not sufficient enough to generate suitable cultivation requirements. Furthermore, process engineering knowledge is inapplicable if it is not linked to the physiological demands of the cells. In the current study, a simple yet comprehensive approach for the characterization and design space prediction of orbitally shaken single-use cultivation systems is presented, including the “classical” Erlenmeyer shake flask, the cylindrical TubeSpin bioreactor and the alternately designed Optimum Growth flask. Cultivations were performed inside and outside the design space to validate the defined culture conditions, so that cultivation success (desired specific growth rates and viable cell densities) could be achieved for each cultivation system.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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