Time efficient way to calculate oxygen transfer areas and power input in cylindrical disposable shaken bioreactors

Klöckner, Wolf; Lattermann, Clemens; Pursche, Franz; Büchs, Jochen; Werner, Sören; Eibl, Dieter

doi:10.1002/btpr.1977

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Time efficient way to calculate oxygen transfer areas and power input in cylindrical disposable shaken bioreactors
Authors:	Klöckner, Wolf Lattermann, Clemens Pursche, Franz Büchs, Jochen Werner, Sören Eibl, Dieter
DOI:	10.1002/btpr.1977
Published in:	Biotechnology Progress
Volume(Issue):	30
Issue:	6
Page(s):	1441
Pages to:	1456
Issue Date:	Nov-2014
Publisher / Ed. Institution:	Wiley
Publisher / Ed. Institution:	Weinheim
ISSN:	8756-7938 1520-6033
Language:	English
Subject (DDC):	660: Chemical engineering
Abstract:	Disposable orbitally shaken bioreactors are a promising alternative to stirred or wave agitated systems for mammalian and plant cell cultivation, because they provide a homogeneous and well defined liquid distribution together with a simple and cost-efficient design. Cultivation conditions in the surface-aerated bioreactors are mainly affected by the size of the volumetric oxygen transfer area (a) and the volumetric power input (P/VL) that both result from the liquid distribution during shaking. Since Computational Fluid Dynamics (CFD)-commonly applied to simulate the liquid distribution in such bioreactors-needs high computing power, this technique is poorly suited to investigate the influence of many different operating conditions in various scales. Thus, the aim of this paper is to introduce a new mathematical model for calculating the values of a and P/VL for liquids with water-like viscosities. The model equations were derived from the balance of centrifugal and gravitational forces exerted during shaking. A good agreement was found among calculated values for a and P/VL, CFD simulation values and empirical results. The newly proposed model enables a time efficient way to calculate the oxygen transfer areas and power input for various shaking frequencies, filling volumes and shaking and reactor diameters. All these parameters can be calculated fast and with little computing power.
URI:	https://digitalcollection.zhaw.ch/handle/11475/2325
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Klöckner, W., Lattermann, C., Pursche, F., Büchs, J., Werner, S., & Eibl, D. (2014). Time efficient way to calculate oxygen transfer areas and power input in cylindrical disposable shaken bioreactors. Biotechnology Progress, 30(6), 1441–1456. https://doi.org/10.1002/btpr.1977

Klöckner, W. et al. (2014) ‘Time efficient way to calculate oxygen transfer areas and power input in cylindrical disposable shaken bioreactors’, Biotechnology Progress, 30(6), pp. 1441–1456. Available at: https://doi.org/10.1002/btpr.1977.

W. Klöckner, C. Lattermann, F. Pursche, J. Büchs, S. Werner, and D. Eibl, “Time efficient way to calculate oxygen transfer areas and power input in cylindrical disposable shaken bioreactors,” Biotechnology Progress, vol. 30, no. 6, pp. 1441–1456, Nov. 2014, doi: 10.1002/btpr.1977.

KLÖCKNER, Wolf, Clemens LATTERMANN, Franz PURSCHE, Jochen BÜCHS, Sören WERNER und Dieter EIBL, 2014. Time efficient way to calculate oxygen transfer areas and power input in cylindrical disposable shaken bioreactors. Biotechnology Progress. November 2014. Bd. 30, Nr. 6, S. 1441–1456. DOI 10.1002/btpr.1977

Klöckner, Wolf, Clemens Lattermann, Franz Pursche, Jochen Büchs, Sören Werner, and Dieter Eibl. 2014. “Time Efficient Way to Calculate Oxygen Transfer Areas and Power Input in Cylindrical Disposable Shaken Bioreactors.” Biotechnology Progress 30 (6): 1441–56. https://doi.org/10.1002/btpr.1977.

Klöckner, Wolf, et al. “Time Efficient Way to Calculate Oxygen Transfer Areas and Power Input in Cylindrical Disposable Shaken Bioreactors.” Biotechnology Progress, vol. 30, no. 6, Nov. 2014, pp. 1441–56, https://doi.org/10.1002/btpr.1977.