| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | A shaken disposable bioreaktor system for controlled insect cell cultivations at millilitre-scale |
| Autor/-in: | Ries, Christoph John, Gernot John, Corinne Eibl-Schindler, Regine Eibl, Dieter |
| DOI: | 10.1002/elsc.200900040 |
| Erschienen in: | Engineering in Life Sciences |
| Band(Heft): | 10 |
| Heft: | 1 |
| Seite(n): | 75 |
| Seiten bis: | 79 |
| Erscheinungsdatum: | Feb-2010 |
| Verlag / Hrsg. Institution: | Wiley |
| Verlag / Hrsg. Institution: | Weinheim |
| ISSN: | 1618-0240 1618-2863 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 660: Technische Chemie |
| Zusammenfassung: | While wave-mixed and stirred bag bioreactors are common devices for rapid, safe insect cell culture-based production at liter-scale, orbitally shaken disposable flasks are mainly used for screening studies at milliliter-scale. In contrast to the two aforementioned bag bioreactor types, which can be operated with standard or disposable sensors, shaker flasks have not been instrumented until recently. The combination of 250 mL disposable shake flasks with PreSens's Shake Flask Reader enables both pH and dissolved oxygen to be measured, as well as allowing characterization of oxygen mass transfer. Volumetric oxygen transfer coefficients (kLa-values) for PreSens 250 mL disposable shake flasks, which were determined for the first time in insect cell culture medium at varying culture volumes and shaker frequencies, ranged between 4.4 and 37.9/h. Moreover, it was demonstrated that online monitoring of dissolved oxygen in shake flasks is relevant for limitation-free growth of insect cells up to high cell densities in batch mode (1.6×107 cells/mL) and for the efficient expression of an intracellular model protein. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/2319 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Ries, C., John, G., John, C., Eibl-Schindler, R., & Eibl, D. (2010). A shaken disposable bioreaktor system for controlled insect cell cultivations at millilitre-scale. Engineering in Life Sciences, 10(1), 75–79. https://doi.org/10.1002/elsc.200900040
Ries, C. et al. (2010) ‘A shaken disposable bioreaktor system for controlled insect cell cultivations at millilitre-scale’, Engineering in Life Sciences, 10(1), pp. 75–79. Available at: https://doi.org/10.1002/elsc.200900040.
C. Ries, G. John, C. John, R. Eibl-Schindler, and D. Eibl, “A shaken disposable bioreaktor system for controlled insect cell cultivations at millilitre-scale,” Engineering in Life Sciences, vol. 10, no. 1, pp. 75–79, Feb. 2010, doi: 10.1002/elsc.200900040.
RIES, Christoph, Gernot JOHN, Corinne JOHN, Regine EIBL-SCHINDLER und Dieter EIBL, 2010. A shaken disposable bioreaktor system for controlled insect cell cultivations at millilitre-scale. Engineering in Life Sciences. Februar 2010. Bd. 10, Nr. 1, S. 75–79. DOI 10.1002/elsc.200900040
Ries, Christoph, Gernot John, Corinne John, Regine Eibl-Schindler, and Dieter Eibl. 2010. “A Shaken Disposable Bioreaktor System for Controlled Insect Cell Cultivations at Millilitre-Scale.” Engineering in Life Sciences 10 (1): 75–79. https://doi.org/10.1002/elsc.200900040.
Ries, Christoph, et al. “A Shaken Disposable Bioreaktor System for Controlled Insect Cell Cultivations at Millilitre-Scale.” Engineering in Life Sciences, vol. 10, no. 1, Feb. 2010, pp. 75–79, https://doi.org/10.1002/elsc.200900040.
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