Title: Mass propagation of Helianthus annuus suspension cells in orbitally shaken bioreactors : improved growth rate in single-use bag bioreactors
Authors : Werner, Sören
Greulich, Judith
Geipel, Katja
Steingroewer, Juliane
Bley, Thomas
Eibl, Dieter
Published in : Engineering in Life Sciences
Volume(Issue) : 14
Issue : 6
Pages : 676
Pages to: 684
Publisher / Ed. Institution : Wiley VCH
Publisher / Ed. Institution: Weinheim, Deutschland
Issue Date: 13-Nov-2014
Language : Englisch / English
Subjects : Plant cell suspension culture; Specific oxygen transfer coefficient; Mixing time; Orbitally shaken bag bioreactor
Subject (DDC) : 660: Technische Chemie
Abstract: Stirred tank-bioreactors made of glass or steel, wave-mixed and orbitally shaken bag bioreactors have all proven to be suitable for the rapid development and commercial production of bioactive compounds with plant cell suspensions. Although these bag bioreactors are characterized by reduced foam formation and less flotation in comparison to stirred systems, their power input is limited. Engineering parameters such as mixing time, oxygen transfer and power input are dependent on the viscosity of the liquid and thus, investigations with plant cell suspensions are necessary. However, to save time and achieve better controllability, sodium carboxymethyl cellulose (Na-CMC) solutions in concentrations ranging from 1 to 20 g·L−1, with viscosities of between 0.005 and 0.4 Pa, were identified as appropriate model systems for mimicking plant cell suspensions with packed cell volumes (PCV) of between 30 and 70 % and similar viscosities. The current study has shown that it is possible to transfer a Helianthus annuus cell suspension process from an orbitally shaken CultiBag RM 1 L to a CultiBag RM with a 10 L working volume by adjusting the operating parameters to achieve a constant kLa value. A maximum specific growth rate μmax of around 0.25 d−1 was achieved, which corresponds to optimized data for shake flasks and even exceeds the growth rate for stirred glass-bioreactors.
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Chemie und Biotechnologie (ICBT)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI : 10.1002/elsc.201400024
ISSN: 1618-0240
URI: https://digitalcollection.zhaw.ch/handle/11475/2312
Appears in Collections:Publikationen Life Sciences und Facility Management

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