1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSchirmaier, Carmen-
dc.contributor.authorJossen, Valentin-
dc.contributor.authorKaiser, Stephan C.-
dc.contributor.authorJüngerkeks, Frank-
dc.contributor.authorSilke, Brill-
dc.contributor.authorSafavi-Nab, Agnieszka-
dc.contributor.authorSiehoff, Ann-
dc.contributor.authorvan den Bos, Christian-
dc.contributor.authorEibl, Dieter-
dc.contributor.authorEibl-Schindler, Regine-
dc.date.accessioned2018-01-30T14:49:54Z-
dc.date.available2018-01-30T14:49:54Z-
dc.date.issued2014-05-
dc.identifier.issn1618-0240de_CH
dc.identifier.issn1618-2863de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2309-
dc.description.abstractSuspension cultures, in which human mesenchymal stem cells (hMSCs) are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines. hMSCs are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. For low-serum conditions (5%) and different types of stirred single-use bioreactors, a suspension criteria-based approach for expanding human adipose tissue-derived mesenchymal stem cells (hASCs) from mL- to pilot scale was successfully developed. For process scale-up, experimental and numerical investigations were performed to (1) predict optimum impeller speeds, (2) determine the main engineering parameters (local shear stress, turbulent dissipation rate, Kolmogorov microscale), and (3) verify suspension criteria NS1 and NS1u for rapid process transfer from 100 mL to 2 L and 35 L cultures. Using optimized mediummicrocarrier combinations as well as NS1 and NS1u as scale-up factors, total hASC quantities between 3 •107 (100 mL scale) and 1 • 1010 (35 L scale) were obtained. The cell quantities obtained are the highest reported to date for scalable single-use bioreactors under low-serum conditions.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofEngineering in Life Sciencesde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleScale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditionsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.publisher.placeWeinheimde_CH
dc.identifier.doi10.1002/elsc.201300134de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end303de_CH
zhaw.pages.start292de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume14de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.
Show simple item record
Schirmaier, C., Jossen, V., Kaiser, S. C., Jüngerkeks, F., Silke, B., Safavi-Nab, A., Siehoff, A., van den Bos, C., Eibl, D., & Eibl-Schindler, R. (2014). Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions. Engineering in Life Sciences, 14(3), 292–303. https://doi.org/10.1002/elsc.201300134
Schirmaier, C. et al. (2014) ‘Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions’, Engineering in Life Sciences, 14(3), pp. 292–303. Available at: https://doi.org/10.1002/elsc.201300134.
C. Schirmaier et al., “Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions,” Engineering in Life Sciences, vol. 14, no. 3, pp. 292–303, May 2014, doi: 10.1002/elsc.201300134.
SCHIRMAIER, Carmen, Valentin JOSSEN, Stephan C. KAISER, Frank JÜNGERKEKS, Brill SILKE, Agnieszka SAFAVI-NAB, Ann SIEHOFF, Christian VAN DEN BOS, Dieter EIBL und Regine EIBL-SCHINDLER, 2014. Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions. Engineering in Life Sciences. Mai 2014. Bd. 14, Nr. 3, S. 292–303. DOI 10.1002/elsc.201300134
Schirmaier, Carmen, Valentin Jossen, Stephan C. Kaiser, Frank Jüngerkeks, Brill Silke, Agnieszka Safavi-Nab, Ann Siehoff, Christian van den Bos, Dieter Eibl, and Regine Eibl-Schindler. 2014. “Scale-up of Adipose Tissue-Derived Mesenchymal Stem Cell Production in Stirred Single-Use Bioreactors under Low-Serum Conditions.” Engineering in Life Sciences 14 (3): 292–303. https://doi.org/10.1002/elsc.201300134.
Schirmaier, Carmen, et al. “Scale-up of Adipose Tissue-Derived Mesenchymal Stem Cell Production in Stirred Single-Use Bioreactors under Low-Serum Conditions.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 292–303, https://doi.org/10.1002/elsc.201300134.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.