Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions
Authors : Schirmaier, Carmen
Jossen, Valentin
Kaiser, Stephan C.
Jüngerkeks, Frank
Silke, Brill
Safavi-Nab, Agnieszka
Siehoff, Ann
van den Bos, Christian
Eibl, Dieter
Eibl-Schindler, Regine
Published in : Engineering in Life Sciences
Volume(Issue) : 14
Issue : 3
Pages : 292
Pages to: 303
Publisher / Ed. Institution : Wiley
Publisher / Ed. Institution: Weinheim
Issue Date: May-2014
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 660: Chemical engineering
Abstract: Suspension cultures, in which human mesenchymal stem cells (hMSCs) are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines. hMSCs are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. For low-serum conditions (5%) and different types of stirred single-use bioreactors, a suspension criteria-based approach for expanding human adipose tissue-derived mesenchymal stem cells (hASCs) from mL- to pilot scale was successfully developed. For process scale-up, experimental and numerical investigations were performed to (1) predict optimum impeller speeds, (2) determine the main engineering parameters (local shear stress, turbulent dissipation rate, Kolmogorov microscale), and (3) verify suspension criteria NS1 and NS1u for rapid process transfer from 100 mL to 2 L and 35 L cultures. Using optimized mediummicrocarrier combinations as well as NS1 and NS1u as scale-up factors, total hASC quantities between 3 •107 (100 mL scale) and 1 • 1010 (35 L scale) were obtained. The cell quantities obtained are the highest reported to date for scalable single-use bioreactors under low-serum conditions.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.1002/elsc.201300134
ISSN: 1618-0240
URI: https://digitalcollection.zhaw.ch/handle/11475/2309
Appears in Collections:Publikationen Life Sciences und Facility Management

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