Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions |
Authors: | Schirmaier, Carmen Jossen, Valentin Kaiser, Stephan C. Jüngerkeks, Frank Silke, Brill Safavi-Nab, Agnieszka Siehoff, Ann van den Bos, Christian Eibl, Dieter Eibl-Schindler, Regine |
DOI: | 10.1002/elsc.201300134 |
Published in: | Engineering in Life Sciences |
Volume(Issue): | 14 |
Issue: | 3 |
Page(s): | 292 |
Pages to: | 303 |
Issue Date: | May-2014 |
Publisher / Ed. Institution: | Wiley |
Publisher / Ed. Institution: | Weinheim |
ISSN: | 1618-0240 1618-2863 |
Language: | English |
Subject (DDC): | 660: Chemical engineering |
Abstract: | Suspension cultures, in which human mesenchymal stem cells (hMSCs) are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines. hMSCs are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. For low-serum conditions (5%) and different types of stirred single-use bioreactors, a suspension criteria-based approach for expanding human adipose tissue-derived mesenchymal stem cells (hASCs) from mL- to pilot scale was successfully developed. For process scale-up, experimental and numerical investigations were performed to (1) predict optimum impeller speeds, (2) determine the main engineering parameters (local shear stress, turbulent dissipation rate, Kolmogorov microscale), and (3) verify suspension criteria NS1 and NS1u for rapid process transfer from 100 mL to 2 L and 35 L cultures. Using optimized mediummicrocarrier combinations as well as NS1 and NS1u as scale-up factors, total hASC quantities between 3 •107 (100 mL scale) and 1 • 1010 (35 L scale) were obtained. The cell quantities obtained are the highest reported to date for scalable single-use bioreactors under low-serum conditions. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/2309 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
There are no files associated with this item.
Show full item record
Schirmaier, C., Jossen, V., Kaiser, S. C., Jüngerkeks, F., Silke, B., Safavi-Nab, A., Siehoff, A., van den Bos, C., Eibl, D., & Eibl-Schindler, R. (2014). Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions. Engineering in Life Sciences, 14(3), 292–303. https://doi.org/10.1002/elsc.201300134
Schirmaier, C. et al. (2014) ‘Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions’, Engineering in Life Sciences, 14(3), pp. 292–303. Available at: https://doi.org/10.1002/elsc.201300134.
C. Schirmaier et al., “Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions,” Engineering in Life Sciences, vol. 14, no. 3, pp. 292–303, May 2014, doi: 10.1002/elsc.201300134.
SCHIRMAIER, Carmen, Valentin JOSSEN, Stephan C. KAISER, Frank JÜNGERKEKS, Brill SILKE, Agnieszka SAFAVI-NAB, Ann SIEHOFF, Christian VAN DEN BOS, Dieter EIBL und Regine EIBL-SCHINDLER, 2014. Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions. Engineering in Life Sciences. Mai 2014. Bd. 14, Nr. 3, S. 292–303. DOI 10.1002/elsc.201300134
Schirmaier, Carmen, Valentin Jossen, Stephan C. Kaiser, Frank Jüngerkeks, Brill Silke, Agnieszka Safavi-Nab, Ann Siehoff, Christian van den Bos, Dieter Eibl, and Regine Eibl-Schindler. 2014. “Scale-up of Adipose Tissue-Derived Mesenchymal Stem Cell Production in Stirred Single-Use Bioreactors under Low-Serum Conditions.” Engineering in Life Sciences 14 (3): 292–303. https://doi.org/10.1002/elsc.201300134.
Schirmaier, Carmen, et al. “Scale-up of Adipose Tissue-Derived Mesenchymal Stem Cell Production in Stirred Single-Use Bioreactors under Low-Serum Conditions.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 292–303, https://doi.org/10.1002/elsc.201300134.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.