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Title: On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur
Authors : Jossen, Valentin
Schirmaier, Carmen
John, Gernot T.
Eibl, Dieter
Eibl-Schindler, Regine
Published in : BIOspektrum
Volume(Issue) : 21
Issue : 4
Pages : 422
Pages to: 424
Publisher / Ed. Institution : Springer
Publisher / Ed. Institution: Berlin
Issue Date: Jun-2015
Language : Deutsch / German
Subject (DDC) : 570: Biologie
Abstract: Spinner flasks are often used for microcarrie-based cultivations of human mesenchymal stem cells (hMSCs). Normally, they are not equipped with pH and dissolved oxygen (DO) probes. This application note describes the cultivation of hMSCs in single-use spinner flasks equipped with optical pH (SP-HP8) and DO (SP-PSt3) sensors for the first time. While reaching peak cell numbers between 4.1 × 107 cells and 5.9 × 107 cells in two cultivation runs, reliable DO and pH data were delivered.
Departement: Life Sciences und Facility Management
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI : 10.1007/s12268-015-0597-4
ISSN: 0947-0867
Appears in Collections:Publikationen Life Sciences und Facility Management

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