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Title: Novel probes for pH and dissolved oxygen measurements in cultivations from millilitre to benchtop scale
Authors : Demuth, Caspar
Varonier, Joel
Jossen, Valentin
Eibl-Schindler, Regine
Eibl, Dieter
Published in : Applied Microbiology and Biotechnology
Volume(Issue) : 100
Issue : 9
Pages : 3853
Pages to: 3863
Publisher / Ed. Institution : Springer
Publisher / Ed. Institution: Berlin
Issue Date: May-2016
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Optical sensors; Single-use cultivation systems; Bioprocess measurement and control; Sensors for pH and dissolved oxygen
Subject (DDC) : 660: Chemical engineering
Abstract: pH value and the concentration of dissolved oxygen (DO) are key parameters to monitor and control cell growth in cultivation studies. Reliable, robust and accurate methods to measure these parameters in cultivation systems in real time guarantee high product yield and quality. This mini-review summarises the current state of the art of pH and DO sensors that are applied to bioprocesses from millilitre to benchtop scale by means of a short introduction on measuring principles and selected applications. Special emphasis is placed on single-use bioreactors, which have been increasingly employed in bioprocess development and production in recent years. Working principles, applications and the particular requirements of sensors in these cultivation systems are given. In such processes, optical sensors for pH and DO are often preferred to electrochemical probes, as they allow semi-invasive measurements and can be miniaturised to micrometre scale or lower. In addition, selected measuring principles of novel sensing technologies for pH and DO are discussed. These include solid-state sensors and miniaturised devices that are not yet commercially available, but show promising characteristics for possible use in bioprocesses in the near future.
Further description : Erworben im Rahmen der Schweizer Nationallizenzen (
Departement: Life Sciences und Facility Management
Publication type: Article in scientific Journal
DOI : 10.1007/s00253-016-7412-0
ISSN: 0175-7598
Restricted until : 2021-01-01
Appears in Collections:Publikationen Life Sciences und Facility Management

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