Title: Fast single-use VLP vaccine productions based on insect cells and the baculovirus expression vector system : influenza as case study
Authors : Eibl-Schindler, Regine
Steiger, Nina
Wellnitz, Sabine
Vicente, Tiago
John, Corinne
Eibl, Dieter
Published in : Disposable Bioreactors II
Pages : 99
Pages to: 125
Editors of the parent work: Eibl, Dieter
Eibl-Schindler, Regine
Publisher / Ed. Institution : Springer
Publisher / Ed. Institution: Berlin
Issue Date: 2014
License (according to publishing contract) : Licence according to publishing contract
Series : Advances in Biochemical Engineering/Biotechnology
Series volume: 138
Type of review: Not specified
Language : English
Subjects : DSP; Influenza; VLP vaccine; Sf-9/BEVS USP; Wave-mixed bioreactor
Subject (DDC) : 615: Pharmacology and therapeutics
Abstract: During the last few years virus like particles (VLPs) have become increasingly interesting for the production of vaccines. This development is explained by their excellent safety profile as well as a significant number of clinical studies showing strong and long-lasting protection. A further reason is the possibility of speeding up VLP vaccine manufacturing by implementing single-use (SU) technology in the case of mammalian and insect-cell-based processes, for which a multitude of SU devices up to middle-volume scale already exist. After briefly introducing the vaccine types and expression systems currently in use, this chapter turns to VLP vaccines and the insect cell/baculovirus expression vector system (IC/BEVS). Based on the main process characteristics and typical process flow of IC/BEVS-based VLP vaccine productions, suitable SU devices and their implementation are addressed. We subsequently report on the successful development of a fast, scalable benchtop production process generating a four-protein component influenza A H1N1 VLP vaccine candidate. This process is based on Spodoptera frugiperda (Sf)-9 cells and combines Redbiotec’s rePAXTM technology with obtainable SU devices for upstream (USP) and downstream processing (DSP).
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Book Part
DOI : 10.1007/10_2013_186
ISBN: 978-3-642-45157-7
978-3-642-45158-4
URI: https://digitalcollection.zhaw.ch/handle/11475/2254
Appears in Collections:Publikationen Life Sciences und Facility Management

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