Title: An approach for scale-up of geometrically dissimilar orbitally shaken single-use bioreactors
Authors : Werner, Sören
Olownia, Johanna
Egger, Daniel
Eibl, Dieter
Published in : Chemie Ingenieur Technik
Volume(Issue) : 85
Issue : 1-2
Pages : 118
Pages to: 126
Publisher / Ed. Institution : Wiley
Publisher / Ed. Institution: Weinheim
Issue Date: Feb-2013
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 660: Chemical engineering
Abstract: An initial approach for scaling up geometrically dissimilar orbitally shaken bioreactors is presented. A novel ShakerBag Option for Multitron Cell shaking incubators allows a rapid increase in working volume from small TubeSpin bioreactors or shake flasks up to 10 L, thus offering a simple and cost-efficient alternative to present systems for scale-up. The engineering parameters for scale-up of the orbitally shaken single-use bags were determined using traditional methods. Modern computational fluid dynamics based methods were used to gain a deeper insight into the fluid flow behavior. Furthermore, mass propagation of plant cell suspensions (Nicotiana tabacum and Vitis vinifera), as well as cell expansion and production of protein complexes using insect cells (Sf-9), show the potential of orbitally shaken single-use bags.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.1002/cite.201200153
ISSN: 0009-286X
URI: https://digitalcollection.zhaw.ch/handle/11475/2241
Appears in Collections:Publikationen Life Sciences und Facility Management

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