Title: Wave-mixed and orbitally shaken single-use photobioreactors for diatom algae propagation
Authors : Lehmann, Nicolai
Rischer, Heiko
Eibl, Dieter
Eibl-Schindler, Regine
Published in : Chemie Ingenieur Technik
Volume(Issue) : 85
Issue : 1-2
Pages : 197
Pages to: 201
Publisher / Ed. Institution : Wiley
Publisher / Ed. Institution: Weinheim
Issue Date: Feb-2013
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Single-use bioreactor; Growth characteristics; Phaeodactylum ricornutum; LED
Subject (DDC) : 660: Chemical engineering
Abstract: Although vertical column, flat-plate, tubular, bubble column and airlift photobioreactors are widely used for the cultivation of diatoms, it has been shown that bubble-induced hydrodynamic stress created in these types of bioreactor can damage cells. Therefore, three single-use surface-aerated bag bioreactors, known for their outstanding results in cultivating shear-sensitive mammalian cells, were for the first time investigated for their suitability in growing the model microalgae Phaeodactylum tricornutum. All of the systems, which were additionally aerated with CO2 and equipped with illumination systems providing different light qualities, guaranteed a 22- to 43-fold increase in cell density within seven days, without any addition of cell protection agents or changes in cell morphology. Maximum cell density and dry biomass were achieved in the orbitally shaken 2D-bag by using cool-white fluorescent tubes.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1002/cite.201200137
ISSN: 0009-286X
URI: https://digitalcollection.zhaw.ch/handle/11475/2233
Appears in Collections:Publikationen Life Sciences und Facility Management

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