1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-22188
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWong, Chee-Wai-
dc.contributor.authorLeGrand, Catherine F.-
dc.contributor.authorKinnear, Beverley F.-
dc.contributor.authorSobota, Radoslaw M.-
dc.contributor.authorRamalingam, Rajkumar-
dc.contributor.authorDye, Danielle E.-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorLane, E. Birgitte-
dc.contributor.authorCoombe, Deirdre R.-
dc.date.accessioned2021-03-26T15:19:38Z-
dc.date.available2021-03-26T15:19:38Z-
dc.date.issued2019-12-06-
dc.identifier.issn2045-2322de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/22188-
dc.description.abstractThe long-term expansion of keratinocytes under conditions that avoid xenogeneic components (i.e. animal serum- and feeder cell-free) generally causes diminished proliferation and increased terminal differentiation. Here we present a culture system free of xenogeneic components that retains the self-renewal capacity of primary human keratinocytes. In vivo the extracellular matrix (ECM) of the tissue microenvironment has a major influence on a cell's fate. We used ECM from human dermal fibroblasts, cultured under macromolecular crowding conditions to facilitate matrix deposition and organisation, in a xenogeneic-free keratinocyte expansion protocol. Phospholipase A2 decellularisation produced ECM whose components resembled the core matrix composition of natural dermis by proteome analyses. Keratinocytes proliferated rapidly on these matrices, retained their small size, expressed p63, lacked keratin 10 and rarely expressed keratin 16. The colony forming efficiency of these keratinocytes was enhanced over that of keratinocytes grown on collagen I, indicating that dermal fibroblast-derived matrices maintain the in vitro expansion of keratinocytes in a stem-like state. Keratinocyte sheets formed on such matrices were multi-layered with superior strength and stability compared to the single-layered sheets formed on collagen I. Thus, keratinocytes expanded using our xenogeneic-free protocol retained a stem-like state, but when triggered by confluence and calcium concentration, they stratified to produce epidermal sheets with a potential clinical use.de_CH
dc.language.isoende_CH
dc.publisherNature Publishing Groupde_CH
dc.relation.ispartofScientific Reportsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectCell culture techniquede_CH
dc.subjectCell differentiationde_CH
dc.subjectCell proliferationde_CH
dc.subjectCultured cellsde_CH
dc.subjectCoculture techniquede_CH
dc.subjectDermisde_CH
dc.subjectExtracellular matrixde_CH
dc.subjectFeeder cellsde_CH
dc.subjectFibroblastsde_CH
dc.subjectHumande_CH
dc.subjectKeratinocytesde_CH
dc.subjectSkin transplantationde_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleIn vitro expansion of keratinocytes on human dermal fibroblast-derived matrix retains their stem-like characteristicsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1038/s41598-019-54793-9de_CH
dc.identifier.doi10.21256/zhaw-22188-
dc.identifier.pmid31811191de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start18561de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume9de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
2019_Wong-et-al_In-Vitro-expansion-of-Keratinocytes-on-Human-Dermal-fibroblast-Derived-Matrix .pdf4.16 MBAdobe PDFThumbnail
View/Open
Show simple item record
Wong, C.-W., LeGrand, C. F., Kinnear, B. F., Sobota, R. M., Ramalingam, R., Dye, D. E., Raghunath, M., Lane, E. B., & Coombe, D. R. (2019). In vitro expansion of keratinocytes on human dermal fibroblast-derived matrix retains their stem-like characteristics. Scientific Reports, 9(1), 18561. https://doi.org/10.1038/s41598-019-54793-9
Wong, C.-W. et al. (2019) ‘In vitro expansion of keratinocytes on human dermal fibroblast-derived matrix retains their stem-like characteristics’, Scientific Reports, 9(1), p. 18561. Available at: https://doi.org/10.1038/s41598-019-54793-9.
C.-W. Wong et al., “In vitro expansion of keratinocytes on human dermal fibroblast-derived matrix retains their stem-like characteristics,” Scientific Reports, vol. 9, no. 1, p. 18561, Dec. 2019, doi: 10.1038/s41598-019-54793-9.
WONG, Chee-Wai, Catherine F. LEGRAND, Beverley F. KINNEAR, Radoslaw M. SOBOTA, Rajkumar RAMALINGAM, Danielle E. DYE, Michael RAGHUNATH, E. Birgitte LANE und Deirdre R. COOMBE, 2019. In vitro expansion of keratinocytes on human dermal fibroblast-derived matrix retains their stem-like characteristics. Scientific Reports. 6 Dezember 2019. Bd. 9, Nr. 1, S. 18561. DOI 10.1038/s41598-019-54793-9
Wong, Chee-Wai, Catherine F. LeGrand, Beverley F. Kinnear, Radoslaw M. Sobota, Rajkumar Ramalingam, Danielle E. Dye, Michael Raghunath, E. Birgitte Lane, and Deirdre R. Coombe. 2019. “In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-like Characteristics.” Scientific Reports 9 (1): 18561. https://doi.org/10.1038/s41598-019-54793-9.
Wong, Chee-Wai, et al. “In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-like Characteristics.” Scientific Reports, vol. 9, no. 1, Dec. 2019, p. 18561, https://doi.org/10.1038/s41598-019-54793-9.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.