Growing human dermal fibroblasts as spheroids renders them susceptible for early expression of pluripotency genes

Lo, Lok Man; Raghunath, Michael; Lee, Kenneth K. H.

doi:10.1002/adbi.201900094

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Growing human dermal fibroblasts as spheroids renders them susceptible for early expression of pluripotency genes
Authors:	Lo, Lok Man Raghunath, Michael Lee, Kenneth K. H.
et. al:	No
DOI:	10.1002/adbi.201900094
Published in:	Advanced Biosystems
Volume(Issue):	3
Issue:	10
Issue Date:	Oct-2019
Publisher / Ed. Institution:	Wiley
ISSN:	2366-7478
Language:	English
Subjects:	RNAseq; Bioinformatics; Human fibroblasts; Pluripotency; Spheroid culture; Animal; Cell culture technique; Cultured cells; Cellular reprogramming; Human; Mice; Pluripotent stem cells; Skin; Fibroblasts; Cellular spheroids; Transcriptome
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	Suspension spheroid cultures of anchorage-dependent cell types have been widely used in cancer and stem cell research, as well as for producing organoids. It is believed that the 3-dimensional spheroid presents cells with a more physiological microenvironment to grow so that they behave more like cells in vivo, which is lacking in conventional 2-dimensional monolayer cultures. Recently, it has been reported that cancer cells grown as spheroids could express stem cell-associated genes. Hence, it is investigated whether normal mouse and human fibroblasts cultured as spheroids could also be induced to express stem cell-associated genes. The transcriptomes of human fibroblasts cultured as a monolayer and spheroids are compared and analyzed using real-time RT-qPCR, RNA-sequencing, and bioinformatics. The results reveal that the spheroid transcriptome resemble somatic cells being reprogramed into stem cells, including the induced expression of stemness-associated genes, increased expression of mesenchymal-to-epithelial transition-associated genes, and decreased expression of epithelial-to-mesenchymal transition-associated genes. In this context, it is hypothesized that during the process of spheroid formation, matrix-cell signaling is lost in favor of cell-cell contact signaling and that this subsequently increases the activity of the PI3K/Akt pathway that then upregulates Tbdx3 and stemness-associated genes.
URI:	https://digitalcollection.zhaw.ch/handle/11475/22185
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Lo, L. M., Raghunath, M., & Lee, K. K. H. (2019). Growing human dermal fibroblasts as spheroids renders them susceptible for early expression of pluripotency genes. Advanced Biosystems, 3(10). https://doi.org/10.1002/adbi.201900094

Lo, L.M., Raghunath, M. and Lee, K.K.H. (2019) ‘Growing human dermal fibroblasts as spheroids renders them susceptible for early expression of pluripotency genes’, Advanced Biosystems, 3(10). Available at: https://doi.org/10.1002/adbi.201900094.

L. M. Lo, M. Raghunath, and K. K. H. Lee, “Growing human dermal fibroblasts as spheroids renders them susceptible for early expression of pluripotency genes,” Advanced Biosystems, vol. 3, no. 10, Oct. 2019, doi: 10.1002/adbi.201900094.

LO, Lok Man, Michael RAGHUNATH und Kenneth K. H. LEE, 2019. Growing human dermal fibroblasts as spheroids renders them susceptible for early expression of pluripotency genes. Advanced Biosystems. Oktober 2019. Bd. 3, Nr. 10. DOI 10.1002/adbi.201900094

Lo, Lok Man, Michael Raghunath, and Kenneth K. H. Lee. 2019. “Growing Human Dermal Fibroblasts as Spheroids Renders Them Susceptible for Early Expression of Pluripotency Genes.” Advanced Biosystems 3 (10). https://doi.org/10.1002/adbi.201900094.

Lo, Lok Man, et al. “Growing Human Dermal Fibroblasts as Spheroids Renders Them Susceptible for Early Expression of Pluripotency Genes.” Advanced Biosystems, vol. 3, no. 10, Oct. 2019, https://doi.org/10.1002/adbi.201900094.