Publication type: Working paper – expertise – study
Title: Automated 3D cell culture using Corning® 96-well spheroid microplates on the Fluent® automation workstation
Authors: Bono, Epifania
et. al: No
Extent: 6
Issue Date: 2020
Publisher / Ed. Institution: Tecan
Language: English
Subjects: Automation; Cell culture; High throughput; Spheroids
Subject (DDC): 610.28: Biomedicine, biomedical engineering
Abstract: In vitro three-dimensional cell models provide more biologically-relevant results than monolayer models for cancer research but the majority of high throughput cell-based screening is still conducted using 2D cultures. The main reason for this is that most available techniques for 3D spheroid generation and quantification are time consuming, tedious and show poor reproducibility. This application note describes the automated production and growth of single spheroids in ultra-low attachment Corning 96-well spheroid microplates on a Fluent Automation Workstation, using a Multiple Channel Arm™ for cell seeding and medium exchange.
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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