Transcriptome analysis of Xanthomonas fragariae in strawberry leaves

Puławska, Joanna; Kałużna, Monika; Warabieda, Wojciech; Pothier, Joël F.; Gétaz, Michael; van der Wolf, Jan M.

doi:10.1038/s41598-020-77612-y

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-21024

Full metadata record

DC Field	Value	Language
dc.contributor.author	Puławska, Joanna	-
dc.contributor.author	Kałużna, Monika	-
dc.contributor.author	Warabieda, Wojciech	-
dc.contributor.author	Pothier, Joël F.	-
dc.contributor.author	Gétaz, Michael	-
dc.contributor.author	van der Wolf, Jan M.	-
dc.date.accessioned	2020-12-11T13:57:45Z	-
dc.date.available	2020-12-11T13:57:45Z	-
dc.date.issued	2020-11-25	-
dc.identifier.issn	2045-2322	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/21024	-
dc.description.abstract	Xanthomonas fragariae is a quarantine bacterial pathogen that causes angular leaf spot on strawberry. The aim of our study was to analyse the mechanism of interaction of this bacterium with its host plant at the transcriptome level. For this purpose, mRNAs of X. fragariae growing in Wilbrink's medium and from infected strawberry cv. Elsanta plants were isolated and sequenced using the Illumina MiSeq platform. The expression profiles of the bacteria in Wilbrink's medium and in planta were very diverse. Of the 3939 CDSs recorded, 1995 had significantly different expression in planta (966 and 1029 genes were down- and upregulated, respectively). Among the genes showing increased expression in planta, those with eggNOG/COG (evolutionary genealogy of genes: Non-supervised Orthologous Groups/Cluster of Orthologous Groups) categories associated with bacterial cell motility, signal transduction, transport and metabolism of inorganic ions and carbohydrates and transcription were overrepresented. Among the genes with the most increased expression in planta, genes primarily associated with flagella synthesis and chemotaxis were found. It is also interesting to note that out of the 31 genes localized on a plasmid, 16 were expressed differently in planta, which may indicate their potential role in plant-pathogen interactions. Many genes with differentiated expression that were localized on chromosome and plasmid encode proteins of unknown function.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Nature Publishing Group	de_CH
dc.relation.ispartof	Scientific Reports	de_CH
dc.rights	http://creativecommons.org/licenses/by/4.0/	de_CH
dc.subject	Xanthomonas	de_CH
dc.subject	Transcriptome	de_CH
dc.subject	RNA-seq	de_CH
dc.subject	Strawberry	de_CH
dc.subject.ddc	570: Biologie	de_CH
dc.title	Transcriptome analysis of Xanthomonas fragariae in strawberry leaves	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Umwelt und Natürliche Ressourcen (IUNR)	de_CH
dc.identifier.doi	10.1038/s41598-020-77612-y	de_CH
dc.identifier.doi	10.21256/zhaw-21024	-
dc.identifier.pmid	33239704	de_CH
zhaw.funding.eu	info:eu-repo/grantAgreement/EC/FP7/613678//Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens/DROPSA	de_CH
zhaw.issue	20582	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	10	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Umweltgenomik	de_CH
zhaw.funding.zhaw	DROPSA – Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens	de_CH
zhaw.author.additional	No	de_CH
zhaw.display.portrait	Yes	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Puławska, J., Kałużna, M., Warabieda, W., Pothier, J. F., Gétaz, M., & van der Wolf, J. M. (2020). Transcriptome analysis of Xanthomonas fragariae in strawberry leaves. Scientific Reports, 10(20582). https://doi.org/10.1038/s41598-020-77612-y

Puławska, J. et al. (2020) ‘Transcriptome analysis of Xanthomonas fragariae in strawberry leaves’, Scientific Reports, 10(20582). Available at: https://doi.org/10.1038/s41598-020-77612-y.

J. Puławska, M. Kałużna, W. Warabieda, J. F. Pothier, M. Gétaz, and J. M. van der Wolf, “Transcriptome analysis of Xanthomonas fragariae in strawberry leaves,” Scientific Reports, vol. 10, no. 20582, Nov. 2020, doi: 10.1038/s41598-020-77612-y.

PUŁAWSKA, Joanna, Monika KAŁUŻNA, Wojciech WARABIEDA, Joël F. POTHIER, Michael GÉTAZ und Jan M. VAN DER WOLF, 2020. Transcriptome analysis of Xanthomonas fragariae in strawberry leaves. Scientific Reports. 25 November 2020. Bd. 10, Nr. 20582. DOI 10.1038/s41598-020-77612-y

Puławska, Joanna, Monika Kałużna, Wojciech Warabieda, Joël F. Pothier, Michael Gétaz, and Jan M. van der Wolf. 2020. “Transcriptome Analysis of Xanthomonas Fragariae in Strawberry Leaves.” Scientific Reports 10 (20582). https://doi.org/10.1038/s41598-020-77612-y.

Puławska, Joanna, et al. “Transcriptome Analysis of Xanthomonas Fragariae in Strawberry Leaves.” Scientific Reports, vol. 10, no. 20582, Nov. 2020, https://doi.org/10.1038/s41598-020-77612-y.