Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata

Bentebibel, Salima; Moyano, Elisabet; Palazón, Javier; Cusidó, Rosa M.; Bonfill, Mercedes; Eibl-Schindler, Regine; Piñol, M. Teresa

doi:10.1002/bit.20321

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata
Authors:	Bentebibel, Salima Moyano, Elisabet Palazón, Javier Cusidó, Rosa M. Bonfill, Mercedes Eibl-Schindler, Regine Piñol, M. Teresa
DOI:	10.1002/bit.20321
Published in:	Biotechnology & Bioengineering
Volume(Issue):	89
Issue:	6
Page(s):	647
Pages to:	655
Issue Date:	2005
Publisher / Ed. Institution:	Wiley
ISSN:	0006-3592 1097-0290
Language:	English
Subject (DDC):	660.6: Biotechnology
Abstract:	Paclitaxel and baccatin III-producing cells of Taxus baccata were immobilized within Ca2+-alginate beads. Under established optimum conditions for the biosynthesis of both taxanes, the yields of paclitaxel and baccatin III in shake-flask cultures of free cells increased by factors of up to 3 and 2, respectively, in the corresponding cultures of immobilized cells. Although the scale-up from shake-flask to bioreactor culture usually results in reduced productivities when both free and immobilized cells were grown in the same optimum conditions in three different bioreactor types (Stirred, Airlift, and Wave) running for 24 days in a batch mode and with the system optimized in each case, there was a considerable increase in the yields of paclitaxel and baccatin III. Among the reactors, the Stirred bioreactor was the most efficient in promoting immobilized cell production of paclitaxel, giving a content of 43.43 mg.L−1 at 16 days of culture, equivalent to a rate of 2.71 mg.L−1.day−1. To our knowledge, the paclitaxel productivity obtained in this study is one of the highest reported so far by academic laboratories for Taxus species cultures in bioreactors.
URI:	https://digitalcollection.zhaw.ch/handle/11475/2098
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Appears in collections:	Publikationen Life Sciences und Facility Management

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Bentebibel, S., Moyano, E., Palazón, J., Cusidó, R. M., Bonfill, M., Eibl-Schindler, R., & Piñol, M. T. (2005). Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata. Biotechnology & Bioengineering, 89(6), 647–655. https://doi.org/10.1002/bit.20321

Bentebibel, S. et al. (2005) ‘Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata’, Biotechnology & Bioengineering, 89(6), pp. 647–655. Available at: https://doi.org/10.1002/bit.20321.

S. Bentebibel et al., “Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata,” Biotechnology & Bioengineering, vol. 89, no. 6, pp. 647–655, 2005, doi: 10.1002/bit.20321.

BENTEBIBEL, Salima, Elisabet MOYANO, Javier PALAZÓN, Rosa M. CUSIDÓ, Mercedes BONFILL, Regine EIBL-SCHINDLER und M. Teresa PIÑOL, 2005. Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata. Biotechnology & Bioengineering. 2005. Bd. 89, Nr. 6, S. 647–655. DOI 10.1002/bit.20321

Bentebibel, Salima, Elisabet Moyano, Javier Palazón, Rosa M. Cusidó, Mercedes Bonfill, Regine Eibl-Schindler, and M. Teresa Piñol. 2005. “Effects of Immobilization by Entrapment in Alginate and Scale-up on Paclitaxel and Baccatin III Production in Cell Suspension Cultures of Taxus Baccata.” Biotechnology & Bioengineering 89 (6): 647–55. https://doi.org/10.1002/bit.20321.

Bentebibel, Salima, et al. “Effects of Immobilization by Entrapment in Alginate and Scale-up on Paclitaxel and Baccatin III Production in Cell Suspension Cultures of Taxus Baccata.” Biotechnology & Bioengineering, vol. 89, no. 6, 2005, pp. 647–55, https://doi.org/10.1002/bit.20321.