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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1552
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dc.contributor.authorFleischli, Franziska-
dc.contributor.authorMathes, Stephanie-
dc.contributor.authorAdlhart, Christian-
dc.date.accessioned2018-01-17T10:42:49Z-
dc.date.available2018-01-17T10:42:49Z-
dc.date.issued2013-09-
dc.identifier.issn0924-2031de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2081-
dc.description.abstractRaman spectroscopy has become a versatile tool for the in vivo charaterisation of skin. Here we describe use of Raman spectroscopy for high resolution optical cross sectioning to resolve skin constituents and administered drugs at the cellular level. Percutaneous penetration is typically studied using permeation cells with biopsies of animals or human skin. Although this technique provides valuable clinical data, little insight is gained in the microstructure of drug penetration (intercellular or transcellular) or in the mode of action of applied vehicles or penetration enhancers. Therefore, a Raman microspectroscopic method was combined with a confocal scanning setup to image the microstructure of commercially available skin models (SkinEthic®) and the spatial distribution of penetrated actives. The models’ microstructure was scanned without any special treatment or environment such as cutting, staining, freezing, or application of vacuum. The non-invasive Raman images reveal the layered structure of stratum corneum. This in particular for lipids while water tends to be more evenly distributed. When penetration of the hydrophilic active glycerol and the lipophilic octyl methoxycinnamate, OMC, was studied, a strong correlation between the local distribution of skin constituents and the hydrophilic/lipophilic character of the active was observed.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofVibrational Spectroscopyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectSkin penetrationde_CH
dc.subjectRaman microscopyde_CH
dc.subjectLabel free imagingde_CH
dc.subjectSkin modelsde_CH
dc.subject.ddc530: Physikde_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleLabel free non-invasive imaging of topically applied actives in reconstructed human epidermis by confocal Raman spectroscopyde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.publisher.placeAmsterdamde_CH
dc.identifier.doi10.21256/zhaw-1552-
dc.identifier.doi10.1016/j.vibspec.2013.05.003de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end33de_CH
zhaw.pages.start29de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume68de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
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Fleischli, F., Mathes, S., & Adlhart, C. (2013). Label free non-invasive imaging of topically applied actives in reconstructed human epidermis by confocal Raman spectroscopy. Vibrational Spectroscopy, 68(1), 29–33. https://doi.org/10.21256/zhaw-1552
Fleischli, F., Mathes, S. and Adlhart, C. (2013) ‘Label free non-invasive imaging of topically applied actives in reconstructed human epidermis by confocal Raman spectroscopy’, Vibrational Spectroscopy, 68(1), pp. 29–33. Available at: https://doi.org/10.21256/zhaw-1552.
F. Fleischli, S. Mathes, and C. Adlhart, “Label free non-invasive imaging of topically applied actives in reconstructed human epidermis by confocal Raman spectroscopy,” Vibrational Spectroscopy, vol. 68, no. 1, pp. 29–33, Sep. 2013, doi: 10.21256/zhaw-1552.
FLEISCHLI, Franziska, Stephanie MATHES und Christian ADLHART, 2013. Label free non-invasive imaging of topically applied actives in reconstructed human epidermis by confocal Raman spectroscopy. Vibrational Spectroscopy. September 2013. Bd. 68, Nr. 1, S. 29–33. DOI 10.21256/zhaw-1552
Fleischli, Franziska, Stephanie Mathes, and Christian Adlhart. 2013. “Label Free Non-Invasive Imaging of Topically Applied Actives in Reconstructed Human Epidermis by Confocal Raman Spectroscopy.” Vibrational Spectroscopy 68 (1): 29–33. https://doi.org/10.21256/zhaw-1552.
Fleischli, Franziska, et al. “Label Free Non-Invasive Imaging of Topically Applied Actives in Reconstructed Human Epidermis by Confocal Raman Spectroscopy.” Vibrational Spectroscopy, vol. 68, no. 1, Sept. 2013, pp. 29–33, https://doi.org/10.21256/zhaw-1552.


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