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|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||A direct interaction of Axonin-1 with Ngcam-related cell adhesion molecule (Nrcam) results in guidance, but not growth of commissural axons|
Stoeckli, Esther T.
Kozlov, Serguei V.
Lane, Robert P.
Suter, Daniel M.
Dreyer, William J.
|Published in:||Journal of Cell Biology|
|Publisher / Ed. Institution:||Rockefeller University Press|
|Subjects:||Animals; Axons; Binding sites; Cell adhesion; Cell adhesion molecules; Chick embryo; Contactin 2; Growth cones; Multigene family; Neural pathways; Protein binding; Recombinant proteins; Spinal cord; Embryonic induction|
|Subject (DDC):||571: Physiology and related subjects|
|Abstract:||An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Organisational Unit:||General Secretary's Office|
|Appears in Collections:||Publikationen Rektorat und Ressorts|
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|2000_Fitzli_etal_Direct-interaction-of-Axonin-1_JCB.pdf||12.72 MB||Adobe PDF|
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