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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Host–pathogen interactions between Xanthomonas fragariae and its host Fragaria × ananassa investigated with a dual RNA-Seq analysis
Authors: Gétaz, Michael
Puławska, Joanna
Smits, Theo H.M.
Pothier, Joël F.
et. al: No
DOI: 10.3390/microorganisms8081253
Published in: Microorganisms
Volume(Issue): 8
Issue: 8
Pages: 1253
Issue Date: 18-Aug-2020
Publisher / Ed. Institution: MDPI
ISSN: 2076-2607
Language: English
Subjects: Strawberry; Plant inoculation; Transcriptome; RNA-sequencing; Virulence factor
Subject (DDC): 572: Biochemistry
Abstract: Strawberry is economically important and widely grown, but susceptible to a large variety of phytopathogenic organisms. Among them, Xanthomonas fragariae is a quarantine bacterial pathogen threatening strawberry productions by causing angular leaf spots. Using whole transcriptome sequencing, the gene expression of both plant and bacteria in planta was analyzed at two time points, 12 and 29 days post inoculation, in order to compare the pathogen and host response between the stages of early visible and of well-developed symptoms. Among 28,588 known genes in strawberry and 4046 known genes in X. fragariae expressed at both time points, a total of 361 plant and 144 bacterial genes were significantly differentially expressed, respectively. The identified higher expressed genes in the plants were pathogen-associated molecular pattern receptors and pathogenesis-related thaumatin encoding genes, whereas the more expressed early genes were related to chloroplast metabolism as well as photosynthesis related coding genes. Most X. fragariae genes involved in host interaction, recognition, and pathogenesis were lower expressed at late-phase infection. This study gives a first insight into the interaction of X. fragariae with its host. The strawberry plant changed gene expression in order to consistently adapt its metabolism with the progression of infection.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project: DROPSA: Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens
Appears in collections:Publikationen Life Sciences und Facility Management

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