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dc.contributor.authorEscursell, Mireia Marcé-
dc.contributor.authorRoschi, Alexia-
dc.contributor.authorSmits, Theo-
dc.contributor.authorRezzonico, Fabio-
dc.date.accessioned2020-07-27T07:50:55Z-
dc.date.available2020-07-27T07:50:55Z-
dc.date.issued2020-07-16-
dc.identifier.issn1125-4653de_CH
dc.identifier.issn2239-7264de_CH
dc.identifier.urihttps://rdcu.be/b5Fvfde_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/20295-
dc.description.abstractErwinia amylovora can rapidly become highly resistant to applications of the antibiotic streptomycin through a single nucleotide mutation in the rpsL gene, which causes an amino acid substitution that prevents inhibitory binding of streptomycin while preserving the functionality of the ribosome. Several viable mutations are described in the literature, but screenings in the orchards consistently revealed that the one leading to the substitution of lysin by arginine at position 43 (K43R) is by far the most abundant. In this work, we tested the rate of emergence of the various spontaneous mutants, their tolerance to different antibiotic concentrations and their general fitness in competition experiments. Furthermore, we developed a molecular assay based on the SNaPshot™ Multiplex Kit from Thermo Fisher Scientific that allows swift determination of rpsL mutations using a single-base primer extension approach directly from colonies on plate. Or results indicate that the prevalence of variant K43R is largely due to fact that it retains the highest environmental fitness even in absence of antibiotic pressure. Sequence analysis of two resistant colonies that did not respond as expected in the SNaPshot™ assay revealed the existence of two yet undescribed rpsL mutations resulting in conditional-lethal streptomycin-dependent (SmD) phenotypes, which are unable to grow in absence of the antibiotic.de_CH
dc.language.isoende_CH
dc.publisherSpringerde_CH
dc.relation.ispartofJournal of Plant Pathologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectStreptomycin resistancede_CH
dc.subjectFire blightde_CH
dc.subjectSNaPshotde_CH
dc.subjectMutationde_CH
dc.subject.ddc570: Biologiede_CH
dc.titleCharacterization and direct molecular discrimination of rpsL mutations leading to high streptomycin resistance in Erwinia amylovorade_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.1007/s42161-020-00600-8de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.snfIZ08Z0_177515de_CH
zhaw.webfeedHealth Research Hub (LSFM)de_CH
zhaw.webfeedUmweltgenomikde_CH
zhaw.funding.zhawSchutz von Apfelwälderökosystemen in Zentralasien vor dem bakteriellen Pathogen Erwinia amylovora (Feuerbrand)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in Collections:Publikationen Life Sciences und Facility Management

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