Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift
Art der Begutachtung: Peer review (Publikation)
Titel: Characterization and direct molecular discrimination of rpsL mutations leading to high streptomycin resistance in Erwinia amylovora
Autor/-in: Escursell, Mireia Marcé
Roschi, Alexia
Smits, Theo
Rezzonico, Fabio
et. al: No
DOI: 10.1007/s42161-020-00600-8
Erschienen in: Journal of Plant Pathology
Erscheinungsdatum: 16-Jul-2020
Verlag / Hrsg. Institution: Springer
ISSN: 1125-4653
2239-7264
Sprache: Englisch
Schlagwörter: Streptomycin resistance; Fire blight; SNaPshot; Mutation
Fachgebiet (DDC): 570: Biologie
Zusammenfassung: Erwinia amylovora can rapidly become highly resistant to applications of the antibiotic streptomycin through a single nucleotide mutation in the rpsL gene, which causes an amino acid substitution that prevents inhibitory binding of streptomycin while preserving the functionality of the ribosome. Several viable mutations are described in the literature, but screenings in the orchards consistently revealed that the one leading to the substitution of lysin by arginine at position 43 (K43R) is by far the most abundant. In this work, we tested the rate of emergence of the various spontaneous mutants, their tolerance to different antibiotic concentrations and their general fitness in competition experiments. Furthermore, we developed a molecular assay based on the SNaPshot™ Multiplex Kit from Thermo Fisher Scientific that allows swift determination of rpsL mutations using a single-base primer extension approach directly from colonies on plate. Or results indicate that the prevalence of variant K43R is largely due to fact that it retains the highest environmental fitness even in absence of antibiotic pressure. Sequence analysis of two resistant colonies that did not respond as expected in the SNaPshot™ assay revealed the existence of two yet undescribed rpsL mutations resulting in conditional-lethal streptomycin-dependent (SmD) phenotypes, which are unable to grow in absence of the antibiotic.
URI: https://rdcu.be/b5Fvf
https://digitalcollection.zhaw.ch/handle/11475/20295
Volltext Version: Publizierte Version
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publiziert im Rahmen des ZHAW-Projekts: Schutz von Apfelwälderökosystemen in Zentralasien vor dem bakteriellen Pathogen Erwinia amylovora (Feuerbrand)
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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