Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Characterization and direct molecular discrimination of rpsL mutations leading to high streptomycin resistance in Erwinia amylovora
Authors : Escursell, Mireia Marcé
Roschi, Alexia
Smits, Theo
Rezzonico, Fabio
et. al : No
DOI : 10.1007/s42161-020-00600-8
Published in : Journal of Plant Pathology
Issue Date: 16-Jul-2020
Publisher / Ed. Institution : Springer
ISSN: 1125-4653
2239-7264
Language : English
Subjects : Streptomycin resistance; Fire blight; SNaPshot; Mutation
Subject (DDC) : 570: Biology
Abstract: Erwinia amylovora can rapidly become highly resistant to applications of the antibiotic streptomycin through a single nucleotide mutation in the rpsL gene, which causes an amino acid substitution that prevents inhibitory binding of streptomycin while preserving the functionality of the ribosome. Several viable mutations are described in the literature, but screenings in the orchards consistently revealed that the one leading to the substitution of lysin by arginine at position 43 (K43R) is by far the most abundant. In this work, we tested the rate of emergence of the various spontaneous mutants, their tolerance to different antibiotic concentrations and their general fitness in competition experiments. Furthermore, we developed a molecular assay based on the SNaPshot™ Multiplex Kit from Thermo Fisher Scientific that allows swift determination of rpsL mutations using a single-base primer extension approach directly from colonies on plate. Or results indicate that the prevalence of variant K43R is largely due to fact that it retains the highest environmental fitness even in absence of antibiotic pressure. Sequence analysis of two resistant colonies that did not respond as expected in the SNaPshot™ assay revealed the existence of two yet undescribed rpsL mutations resulting in conditional-lethal streptomycin-dependent (SmD) phenotypes, which are unable to grow in absence of the antibiotic.
URI: https://rdcu.be/b5Fvf
https://digitalcollection.zhaw.ch/handle/11475/20295
Fulltext version : Published version
License (according to publishing contract) : Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project : Schutz von Apfelwälderökosystemen in Zentralasien vor dem bakteriellen Pathogen Erwinia amylovora (Feuerbrand)
Appears in Collections:Publikationen Life Sciences und Facility Management

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