Title: Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD
Authors : Schenk, A
Ziegler, LN
Meier, Nadja
Peter, Samuel
Wolfram, Evelyn
Proceedings: Planta Medica International Open
Volume(Issue) : 4
Conference details: 65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017), Basel, September 3 rd to 6th 2017
Publisher / Ed. Institution : Thieme
Issue Date: 24-Oct-2017
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Abstract)
Language : English
Subject (DDC) : 572: Biochemistry
615: Pharmacology and therapeutics
660.6: Biotechnology
Abstract: Using a coreshell column, the new UHPLC-DAD method allows almost a baseline separation of seven sennosides and the monomeric hydroxyanthracene glycosides rhein 8-glycoside and aloe emodin 8-glycoside in senna pod dry extract and in a senna-fig syrup preparation. UV and mass spectrometry studies show that none of the peaks of interest is underlaid by flavonoids, a fact which, in the case of other LC methods for sennosides, requires a sample processing by means of SPE. Sennosides are determined by sennoside B, monomeric hydroyanthracene glycosides by rhein 8-glycoside as external standard. The combined determination of all hyrdoxyanthracene glycosides is important for pharmaceutical stability testing because sennosides are partly degraded to rhein- and aloe emodin 8-glycoside. In the posterior part of the chromatogram the aglycones rhein and aloe emodine can be determined. The complete assay runs 19 min.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Conference Poster
DOI : 10.1055/s-0037-1608215
ISSN: 2509-6656
URI: https://digitalcollection.zhaw.ch/handle/11475/1964
Appears in Collections:Publikationen Life Sciences und Facility Management

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