|Title:||Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD|
|Authors :||Schenk, A|
|Proceedings:||Planta Medica International Open|
|Conference details:||65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017), Basel, September 3 rd to 6th 2017|
|Publisher / Ed. Institution :||Thieme|
|Language :||Englisch / English|
|Subject (DDC) :||572: Biochemie|
615: Pharmakologie und Therapeutik
|Abstract:||Using a coreshell column, the new UHPLC-DAD method allows almost a baseline separation of seven sennosides and the monomeric hydroxyanthracene glycosides rhein 8-glycoside and aloe emodin 8-glycoside in senna pod dry extract and in a senna-fig syrup preparation. UV and mass spectrometry studies show that none of the peaks of interest is underlaid by flavonoids, a fact which, in the case of other LC methods for sennosides, requires a sample processing by means of SPE. Sennosides are determined by sennoside B, monomeric hydroyanthracene glycosides by rhein 8-glycoside as external standard. The combined determination of all hyrdoxyanthracene glycosides is important for pharmaceutical stability testing because sennosides are partly degraded to rhein- and aloe emodin 8-glycoside. In the posterior part of the chromatogram the aglycones rhein and aloe emodine can be determined. The complete assay runs 19 min.|
|Departement:||Life Sciences und Facility Management|
|Organisational Unit:||Institut für Chemie und Biotechnologie (ICBT)|
|Publication type:||Konferenz: Poster / Conference Poster|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.