Title: Development of an HPTLC identification method in new Ph Eur monograph for Rubi ideae folium (raspberry leaves)
Authors : Wolfram, Evelyn
Meier, Nadja
Peter, Samuel
Meier, Beat
Proceedings: Planta Medica International Open
Volume(Issue) : 4
Conference details: 65th Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA), Basel, September 3 rd to 6th 2017
Publisher / Ed. Institution : Thieme
Issue Date: 2017
Language : Englisch / English
Subject (DDC) : 572: Biochemie
615: Pharmakologie und Therapeutik
660.6: Biotechnologie
Abstract: Rubi ideae folium, a traditional remedy for pain relief and smooth muscle relacation especially as support before and during child delivery has been recently assessed by the HMPC [1] and has not yet been monographed in the European Pharmacopeia. The development of the valid quality control methods for identification, contaminants and content assay is ongoing. This study developed an HPTLC method according to the novel. Four different mobile phases have been tested until the ideal mix of anhydrous formic acid R, water R, ethylacetate R (10:10:80, V/V/V) was chosen for good fingerprint separation. Hyperosid (2 mg/10 mL in methanol) und Chlorogenic acid (2 mg/10 mL in methanol) serve as System Suitability Solution for SST and Rutin (2 mg in 10 mL Methanol) applied as Rf reference value. The results are a clear and well separated fingerprint – the prominent yellow band has not yet clearly identified. Results from TLC-ESI-MS and TLC-MALDI analysis give mass values which suggest the presence of quercitin glucoronide. However these results need to be confirmed by further in depth isolation and structure elucidation invetigations. [1] EMA (2012), Assessment report on Rubus idaeus L, folium. Commitee on Herbal medicinal products. EMA/HMPC/44209/2012 [2] EDQM (2017), 2.8.25. High performance thin-layer chromatography of herbal drugs and herbal drug preparations. Ph Eur Edition 9.0
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Chemie und Biotechnologie (ICBT)
Publication type: Konferenz: Poster / Conference Poster
DOI : 10.1055/s-0037-1608528
ISSN: 2509-6656
URI: https://digitalcollection.zhaw.ch/handle/11475/1960
Appears in Collections:Publikationen Life Sciences und Facility Management

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