Publikationstyp: Konferenz: Sonstiges
Art der Begutachtung: Peer review (Abstract)
Titel: Enhanced process insights in shake flask cultivations
Autor/-in: John, Gernot. T
Maschke, Rüdiger
et. al: No
Angaben zur Konferenz: BioTech 2019 : Biopharmaceutical Manufacturing and Single-Use Technologies, Wädenswil, 2-4 July 2019
Erscheinungsdatum: Jul-2019
Sprache: Englisch
Fachgebiet (DDC): 540: Chemie
Zusammenfassung: While sophisticated small-scale cultivation systems came to the market in the last few years, shake flasks maintain the economic alternative widely used in industry and academia. Yet, these vessels lack the sensors like turbidity, oxygen concentration and pH-values. Here we show how to overcome these hurdles. The first part covers measurement improvements for shake flasks with focus on back-scattered light for biomass growth monitoring and the utilization of sensor spots for a deeper insight into the “black box” shake flasks. We used plant cells as their growth often can’t be accurately monitored by simple offline OD measurements. The sensor spot measurements are further used to detect process events, enabling the prediction of metabolite depletion. In the second part, soft sensors based on engineering and biological characterizations of these vessels are introduced and explained, enabling researchers to predict process values and estimate growth curves.
Volltext Version: Publizierte Version
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Chemie und Biotechnologie (ICBT)
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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