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dc.contributor.authorSchirmer, Cedric-
dc.contributor.authorMüller, Jan-
dc.contributor.authorSteffen, Nina-
dc.contributor.authorWerner, Sören-
dc.contributor.authorEibl-Schindler, Regine-
dc.contributor.authorEibl, Dieter-
dc.date.accessioned2020-01-30T13:23:32Z-
dc.date.available2020-01-30T13:23:32Z-
dc.date.issued2020-
dc.identifier.isbn978-1-0716-0190-7de_CH
dc.identifier.isbn978-1-0716-0191-4de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/19321-
dc.description.abstractSingle-use bioreactors have increasingly been used in recent years, for both research and development as well as industrial production, especially in mammalian cell-based processes. Among the numerous single-use bioreactors available today, wave-mixed bags and stirred systems dominate. Wave-mixed single-use bioreactors are the system of choice for inoculum production, while stirred single-use bioreactors are most often preferred for antibody expression. For this reason, the present chapter describes protocols instructing the reader to use the wave-mixed BIOSTAT® RM 50 for cell expansion and to produce a monoclonal antibody (mAb) in Pall's Allegro™ STR 200 at pilot scale for the first time. All methods described are based on a Chinese hamster ovary (CHO) suspension cell line expressing a recombinant immunoglobulin G (IgG).de_CH
dc.language.isoende_CH
dc.publisherSpringerde_CH
dc.relation.ispartofAnimal cell biotechnology : methods and protocolsde_CH
dc.relation.ispartofseriesMethods in Molecular Biologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectCHO cell expansionde_CH
dc.subjectFed-batchde_CH
dc.subjectIgG productionde_CH
dc.subjectStirred single-use bioreactorde_CH
dc.subjectWave-mixed single-use bioreactorde_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleHow to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scalede_CH
dc.typeBuchbeitragde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.publisher.placeNew Yorkde_CH
dc.identifier.doi10.1007/978-1-0716-0191-4_10de_CH
dc.identifier.pmid31858468de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end186de_CH
zhaw.pages.start169de_CH
zhaw.parentwork.editorPörtner, Ralf-
zhaw.publication.statuspublishedVersionde_CH
zhaw.series.number2095de_CH
zhaw.publication.reviewEditorial reviewde_CH
zhaw.webfeedVerfahrenstechnikde_CH
zhaw.author.additionalNode_CH
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Schirmer, C., Müller, J., Steffen, N., Werner, S., Eibl-Schindler, R., & Eibl, D. (2020). How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale. In R. Pörtner (Ed.), Animal cell biotechnology : methods and protocols (pp. 169–186). Springer. https://doi.org/10.1007/978-1-0716-0191-4_10
Schirmer, C. et al. (2020) ‘How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale’, in R. Pörtner (ed.) Animal cell biotechnology : methods and protocols. New York: Springer, pp. 169–186. Available at: https://doi.org/10.1007/978-1-0716-0191-4_10.
C. Schirmer, J. Müller, N. Steffen, S. Werner, R. Eibl-Schindler, and D. Eibl, “How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale,” in Animal cell biotechnology : methods and protocols, R. Pörtner, Ed. New York: Springer, 2020, pp. 169–186. doi: 10.1007/978-1-0716-0191-4_10.
SCHIRMER, Cedric, Jan MÜLLER, Nina STEFFEN, Sören WERNER, Regine EIBL-SCHINDLER und Dieter EIBL, 2020. How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale. In: Ralf PÖRTNER (Hrsg.), Animal cell biotechnology : methods and protocols. New York: Springer. S. 169–186. ISBN 978-1-0716-0190-7
Schirmer, Cedric, Jan Müller, Nina Steffen, Sören Werner, Regine Eibl-Schindler, and Dieter Eibl. 2020. “How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.” In Animal Cell Biotechnology : Methods and Protocols, edited by Ralf Pörtner, 169–86. New York: Springer. https://doi.org/10.1007/978-1-0716-0191-4_10.
Schirmer, Cedric, et al. “How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.” Animal Cell Biotechnology : Methods and Protocols, edited by Ralf Pörtner, Springer, 2020, pp. 169–86, https://doi.org/10.1007/978-1-0716-0191-4_10.


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