Publication type: Book part
Type of review: Editorial review
Title: How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale
Authors: Schirmer, Cedric
Müller, Jan
Steffen, Nina
Werner, Sören
Eibl-Schindler, Regine
Eibl, Dieter
et. al: No
DOI: 10.1007/978-1-0716-0191-4_10
Published in: Animal cell biotechnology : methods and protocols
Editors of the parent work: Pörtner, Ralf
Pages: 169
Pages to: 186
Issue Date: 2020
Series: Methods in Molecular Biology
Series volume: 2095
Publisher / Ed. Institution: Springer
Publisher / Ed. Institution: New York
ISBN: 978-1-0716-0190-7
Language: English
Subjects: CHO cell expansion; Fed-batch; IgG production; Stirred single-use bioreactor; Wave-mixed single-use bioreactor
Subject (DDC): 660: Chemical engineering
Abstract: Single-use bioreactors have increasingly been used in recent years, for both research and development as well as industrial production, especially in mammalian cell-based processes. Among the numerous single-use bioreactors available today, wave-mixed bags and stirred systems dominate. Wave-mixed single-use bioreactors are the system of choice for inoculum production, while stirred single-use bioreactors are most often preferred for antibody expression. For this reason, the present chapter describes protocols instructing the reader to use the wave-mixed BIOSTAT® RM 50 for cell expansion and to produce a monoclonal antibody (mAb) in Pall's Allegro™ STR 200 at pilot scale for the first time. All methods described are based on a Chinese hamster ovary (CHO) suspension cell line expressing a recombinant immunoglobulin G (IgG).
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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