| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Ultrasensitive detection of salmonella and listeria monocytogenes by small-molecule chemiluminescence probes |
| Autor/-in: | Roth-Konforti, Michal Green, Ori Hupfeld, Mario Fieseler, Lars Heinrich, Nadine Ihssen, Julian Vorberg, Raffael Wick, Lukas Spitz, Urs Shabat, Doron |
| et. al: | No |
| DOI: | 10.1002/anie.201904719 |
| Erschienen in: | Angewandte Chemie: International Edition |
| Band(Heft): | 58 |
| Heft: | 30 |
| Seite(n): | 10361 |
| Seiten bis: | 10367 |
| Erscheinungsdatum: | 2019 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 1433-7851 1521-3773 |
| Sprache: | Englisch |
| Schlagwörter: | Listeria; Salmonella; Bacterial probe; Chemiluminescence; Dioxetanes |
| Fachgebiet (DDC): | 660: Technische Chemie |
| Zusammenfassung: | Detection of Salmonella and L. monocytogenes in food samples by current diagnostic methods requires relatively long time to results (2-6 days). Furthermore, the ability to perform environmental monitoring at the factory site for these pathogens is limited due to the need for laboratory facilities. Herein, we report new chemiluminescence probes for the ultrasensitive direct detection of viable pathogenic bacteria. The probes are composed of a bright phenoxy-dioxetane luminophore masked by triggering group, which is activated by a specific bacterial enzyme, and could detect their corresponding bacteria with an LOD value of about 600-fold lower than that of fluorescent probes. Moreover, we were able to detect a minimum of 10 Salmonella cells within 6 h incubation. The assay allows for bacterial enrichment and detection in one test tube without further sample preparation. We anticipate that this design strategy will be used to prepare analogous chemiluminescence probes for other enzymes relevant to specific bacteria detection and point-of-care diagnostics. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/18412 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Lebensmittel- und Getränkeinnovation (ILGI) |
| Publiziert im Rahmen des ZHAW-Projekts: | Nachweis von L. monocytogenes und Salmonella in Lebensmitteln und von Oberflächenabstrichen mittels AquaSparkTM Technologie |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Roth-Konforti, M., Green, O., Hupfeld, M., Fieseler, L., Heinrich, N., Ihssen, J., Vorberg, R., Wick, L., Spitz, U., & Shabat, D. (2019). Ultrasensitive detection of salmonella and listeria monocytogenes by small-molecule chemiluminescence probes. Angewandte Chemie: International Edition, 58(30), 10361–10367. https://doi.org/10.1002/anie.201904719
Roth-Konforti, M. et al. (2019) ‘Ultrasensitive detection of salmonella and listeria monocytogenes by small-molecule chemiluminescence probes’, Angewandte Chemie: International Edition, 58(30), pp. 10361–10367. Available at: https://doi.org/10.1002/anie.201904719.
M. Roth-Konforti et al., “Ultrasensitive detection of salmonella and listeria monocytogenes by small-molecule chemiluminescence probes,” Angewandte Chemie: International Edition, vol. 58, no. 30, pp. 10361–10367, 2019, doi: 10.1002/anie.201904719.
ROTH-KONFORTI, Michal, Ori GREEN, Mario HUPFELD, Lars FIESELER, Nadine HEINRICH, Julian IHSSEN, Raffael VORBERG, Lukas WICK, Urs SPITZ und Doron SHABAT, 2019. Ultrasensitive detection of salmonella and listeria monocytogenes by small-molecule chemiluminescence probes. Angewandte Chemie: International Edition. 2019. Bd. 58, Nr. 30, S. 10361–10367. DOI 10.1002/anie.201904719
Roth-Konforti, Michal, Ori Green, Mario Hupfeld, Lars Fieseler, Nadine Heinrich, Julian Ihssen, Raffael Vorberg, Lukas Wick, Urs Spitz, and Doron Shabat. 2019. “Ultrasensitive Detection of Salmonella and Listeria Monocytogenes by Small-Molecule Chemiluminescence Probes.” Angewandte Chemie: International Edition 58 (30): 10361–67. https://doi.org/10.1002/anie.201904719.
Roth-Konforti, Michal, et al. “Ultrasensitive Detection of Salmonella and Listeria Monocytogenes by Small-Molecule Chemiluminescence Probes.” Angewandte Chemie: International Edition, vol. 58, no. 30, 2019, pp. 10361–67, https://doi.org/10.1002/anie.201904719.
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