Title: Ultrasensitive detection of salmonella and listeria monocytogenes by small-molecule chemiluminescence probes
Authors : Roth-Konforti, Michal
Green, Ori
Hupfeld, Mario
Fieseler, Lars
Heinrich, Nadine
Ihssen, Julian
Vorberg, Raffael
Wick, Lukas
Spitz, Urs
Shabat, Doron
et. al : No
Published in : Angewandte Chemie : international edition
Volume(Issue) : 58
Issue : 30
Pages : 10361
Pages to: 10367
Publisher / Ed. Institution : Wiley
Issue Date: 2019
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subjects : Listeria; Salmonella; Bacterial probe; Chemiluminescence; Dioxetanes
Subject (DDC) : 660: Chemical engineering
Abstract: Detection of Salmonella and L. monocytogenes in food samples by current diagnostic methods requires relatively long time to results (2-6 days). Furthermore, the ability to perform environmental monitoring at the factory site for these pathogens is limited due to the need for laboratory facilities. Herein, we report new chemiluminescence probes for the ultrasensitive direct detection of viable pathogenic bacteria. The probes are composed of a bright phenoxy-dioxetane luminophore masked by triggering group, which is activated by a specific bacterial enzyme, and could detect their corresponding bacteria with an LOD value of about 600-fold lower than that of fluorescent probes. Moreover, we were able to detect a minimum of 10 Salmonella cells within 6 h incubation. The assay allows for bacterial enrichment and detection in one test tube without further sample preparation. We anticipate that this design strategy will be used to prepare analogous chemiluminescence probes for other enzymes relevant to specific bacteria detection and point-of-care diagnostics.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Publication type: Article in scientific journal
DOI : 10.1002/anie.201904719
ISSN: 1433-7851
URI: https://digitalcollection.zhaw.ch/handle/11475/18412
Published as part of the ZHAW project : Nachweis von L. monocytogenes und Salmonella in Lebensmitteln und von Oberflächenabstrichen mittels AquaSparkTM Technologie
Appears in Collections:Publikationen Life Sciences und Facility Management

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