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dc.contributor.authorButtimer, Colin-
dc.contributor.authorBorn, Yannick-
dc.contributor.authorLucid, Alan-
dc.contributor.authorLoessner, Martin J.-
dc.contributor.authorFieseler, Lars-
dc.contributor.authorCoffey, Aidan-
dc.date.accessioned2019-10-16T13:50:15Z-
dc.date.available2019-10-16T13:50:15Z-
dc.date.issued2018-
dc.identifier.issn0923-2508de_CH
dc.identifier.issn1769-7123de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/18411-
dc.description.abstractTo date, a small number of jumbo myoviruses have been reported to possess atypical whisker-like structures along the surface of their contractile tails. Erwinia amylovora phage vB_EamM_Y3 is another example. It possesses a genome of 261,365 kbp with 333 predicted ORFs. Using a combination of BLASTP, Interproscan and HHpred, about 21% of its putative proteins could be assigned functions involved in nucleotide metabolism, DNA replication, virion structure and cell wall degradation. The phage was found to have a signal-arrest-release (SAR) endolysin (Y3_301) possessing a soluble lytic transglycosylase domain. Like other SAR endolysins, inducible expression of Y3_301 caused Escherichia coli lysis, which is dependent on the presence of an N-terminal signal sequence. Phylogenetic analysis showed that its closest relatives are other jumbo phages including Pseudomonas aeruginosa phage PaBG and P. putida phage Lu11, sharing 105 and 87 homologous proteins respectively. Like these phages, Y3 also shares a distant relationship to Ralstonia solanacearum phage ΦRSL1 (sharing 55 homologous proteins). As these phages are unrelated to the Rak2-like group of hairy phages, Y3 along with Lu11 represent a second lineage of hairy myoviruses.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofResearch in Microbiologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectErwinia amylovorade_CH
dc.subjectGenomede_CH
dc.subjectHairy phagede_CH
dc.subjectJumbo bacteriophagede_CH
dc.subjectMyoviridaede_CH
dc.subjectSAR endolysinde_CH
dc.subjectDNA replicationde_CH
dc.subjectDNA, Viralde_CH
dc.subjectEndopeptidasesde_CH
dc.subjectErwinia amylovorade_CH
dc.subjectMyoviridaede_CH
dc.subjectOpen reading framede_CH
dc.subjectPhylogenyde_CH
dc.subjectDNA sequence analysisde_CH
dc.subjectViral proteinsde_CH
dc.subjectVirionde_CH
dc.subjectViral genomede_CH
dc.subject.ddc570: Biologiede_CH
dc.titleErwinia amylovora phage vB_EamM_Y3 represents another lineage of hairy myoviridaede_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Lebensmittel- und Getränkeinnovation (ILGI)de_CH
dc.identifier.doi10.1016/j.resmic.2018.04.006de_CH
dc.identifier.pmid29777834de_CH
zhaw.funding.euNode_CH
zhaw.issue9de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end514de_CH
zhaw.pages.start505de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume169de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.snf310030_156947/1de_CH
zhaw.webfeedMikrobiologiede_CH
zhaw.funding.zhawMechanisms of phage resistance in Erwinia amylovorade_CH
zhaw.author.additionalNode_CH
Appears in Collections:Publikationen Life Sciences und Facility Management

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